LOCUS       sequence-68642-e        1046 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  ecDHFR based destable domain function at low temperature for 
            regulating protein stability in Caenorhabditis elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Cho U, Zimmerman SM, Chen LC, Owen E, Kim JV, Kim SK, Wandless TJ
  TITLE     Rapid and Tunable Control of Protein Stability in Caenorhabditis 
            elegans Using a Small Molecule.
  JOURNAL   PLoS One. 2013 Aug 22;8(8):e72393. doi: 
            10.1371/journal.pone.0072393.
  PUBMED    23991108
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-08-22"; volume: "8(8):e72393. doi"; pages: " 
            10.1371/journal.pone.0072393"
COMMENT     Sequence Label: pBMN YFP-ltDHFR(DD)
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..61
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(62..64,65..67,68..778)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(107..128)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(368..387)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     715..736
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gaccatcctc tagactgccg gatccgccac catgtatccg tacgacgtac cggattatgc
       61 gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      121 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      181 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      241 cctcgtgacc accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
      301 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      361 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      421 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      481 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
      541 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      601 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      661 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      721 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagct
      781 cgagatcagt ctgattgcgg cgttagcggt agatcacgtt atcggcatgg aaaacgccat
      841 gccgtggaac ctgcctgccg atctcgcctg gtttaaacgc aacaccttag ataaacccgt
      901 gattatgggc cgccatacct gggaatcaat cggtcgtccg ttgccaggac gcaaagatat
      961 tatcctcagc agtcaaccga gtacggacga tcgcgtaacg tgggtgaagt cggtggatga
     1021 agccatcgcg gcgtgtggtg acgtac
//
