LOCUS       sequence-68752-e       11019 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length EBA181 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 11019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: EBA181-bio
FEATURES             Location/Qualifiers
     source          1..11019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6399..6454
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6453..6472)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8690..8708)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8776..8880
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8881..8949,8950..9741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9099..9118)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9912..10500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10401..10420
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10654..10671
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10788..10809
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10824..10829,10830..10847,10848..10854)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10862..10878
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10867..10889
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10886..10902
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10886..10902
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccatca gcgaggaata cctgaaagag cggccccagg
     1381 gcctgaacgt ggagacaaac aacaacaata acaataataa caacaataat gccaacagca
     1441 acgacgccat gagcttcgtg aacgaagtga tccggttcat cgagaacgag aaggacgaca
     1501 aagaggacaa gaaagtcaag atcatcagca gacccgtgga gaacaccctg cacagatacc
     1561 ccgtgtccag cttcctgaac atcaagaagt acggccggaa gggcgagtac ctgaaccgga
     1621 acagcttcgt gcagcggagc tacatccggg gctgcaaggg caagagaagc acccacacct
     1681 ggatctgcga gaacaagggc aacaacaaca tctgcatccc cgacagacgg gtgcagctgt
     1741 gcatcaccgc cctgcaggac ctgaagaaca gcggcagcga gacaaccgac cggaagctgc
     1801 tgcgggacaa ggtgttcgac agcgccatgt acgagacaga tctgctgtgg aacaagtacg
     1861 gcttccgggg cttcgacgac ttctgcgacg acgtgaagaa ctcctacctg gactacaagg
     1921 acgtgatctt cggcaccgac ctggacaaga acaatatcgc caaactggtg gaggaaagcc
     1981 tgaagcggtt cttcaagaaa gacagcagcg tgctgaaccc caccgcctgg tggagaagat
     2041 acggcacccg gctgtggaaa accatgatcc agccctacgc ccacctgggc tgcagaaagc
     2101 ccgacgagaa cgagccccag atcaaccggt ggatcctgga atggggcaag tacaactgcc
     2161 ggctgatgaa agagaaagag aagctgctga ccggcgagtg cagcgtgaac cggaagaagt
     2221 ccgactgcag caccggctgc aacaacgagt gctacaccta ccggtccctg atcaaccggc
     2281 agagatacga ggtgtccatc ctgggcaaga aatacatcaa ggtcgtgcgg tacaccatct
     2341 ttcggagaaa gatcgtgcag cccgacaacg ccctggactt cctgaagctg aactgcgccg
     2401 agtgcaagga catcgacttc aagccctttt tcgagttcga gtacggcaag tacgaagaga
     2461 agtgcatgtg ccagagctac atcgacctga agatccagtt caagaacaac gacatctgta
     2521 gcttcaacgc ccagaccgac accgtgtcct ccgacaagcg gttctgcctg gaaaagaaag
     2581 agttcaagcc ctggaagtgc gacaagaact ccttcgagac agtgcaccac aagggcgtgt
     2641 gcgtgagccc tcggagacag ggcttctgcc tgggcaacct gaactatctg ctgaacgacg
     2701 acatctacaa cgtgcacaac agccagctgc tgatcgagat catcatggcc agcaagcagg
     2761 aaggcaagct gctctggaag aagcacggca ccatcctgga taaccagaac gcctgcaagt
     2821 acatcaacga cgcctacgtg gattacaaag acatcgtgat cggcaacgac ctgtggaacg
     2881 acaacaacgc catcaaggtg cagaacaacc tgaacctgat cttcgagcgg aacttcggct
     2941 acaaagtggg ccggaacaag ctgttcaaga ccatcaaaga actgaagaac gtgtggtgga
     3001 ttctgaacag aaacaaagtg tgggagagca tgagatgcgg catcgacgag gtggaccagc
     3061 ggagaaagac atgcgagaga atcgacgagc tggaaaacat gccccagttc ttccggtggt
     3121 tcagccagtg ggctcacttc ttctgcaaag aaaaagagta ctgggagctg aaactgaacg
     3181 acaagtgcac cggcaacaac ggcaagagcc tgtgccagga caagacctgt cagaacgtgt
     3241 gcaccaacat gaactactgg acctacaccc ggaagctggc ctacgagatc cagagcgtga
     3301 agtacgacaa ggacagaaag ctgttctccc tggccaagga caagaacgtg gccaccttcc
     3361 tgaaagagaa cgccaagaac tgcgccaaca tcgactttac caagatcttc gaccagctgg
     3421 acaagctgtt taaagaacgg tgcagctgca tggacaccca ggtcctggaa gtgaagaaca
     3481 aagagatgct gtccatcgac agcaacagcg aggacgccac cgacatcagc gagaagaacg
     3541 gcgaagagga actgtacgtg aaccacaaca gcgtgtccgt ggccagcggc aacaaagaaa
     3601 tcgagaagtc caaggacgag aagcagcccg agaaagaggc caagcagacc aacggcgccc
     3661 tgaccgtgcg gaccgacaag gactccgacc ggaataaggg caaggacacc gccaccgata
     3721 ccaagaacag ccccgagaac ctgaaggtgc aggaacacgg caccaacggc gagacaatca
     3781 aagaggaacc ccccaagctg cccgagagca gcgaaaccct gcagagccag gaacagctgg
     3841 aagccgaggc ccagaagcag aagcaggaag aagaacccaa gaagaaacag gaagaggaac
     3901 ctaaaaagaa gcaggaagag gaacagaagc gggagcagga acagaaacag gaacaggaag
     3961 aagaagaaca gaaacaggaa gaagaacagc agatccagga ccagagccag agcggcctgg
     4021 accagagcag caaagtgggc gtggcctccg agcagaacga gatcagcagc ggccaggaac
     4081 agaacgtcaa gagcagcagc cccgaggtgg tgccccagga aaccacaagc gagaacggcg
     4141 ccagccagga caccaagatc agctccacag agcccaacga gaacagcgtg gtggaccggg
     4201 ccaccgacag catgaacctg gaccccgaga aggtgcacaa cgagaatatg gccgacccca
     4261 acaccaacac cgagcccgac gccagcctga aggacgataa gaaagaggtg gacgacgcca
     4321 agaaagaact gcagagcacc gtgtcccgga tcgagtccaa cgagcaggac gtgcagagca
     4381 caccccccga ggatacccct accgtggagg gcaaagtggg ggacaaggcc gagatgctga
     4441 ccagccccca cgccacagac aactccgaga gcgagtccgg cctgaaccct accgacgaca
     4501 tcaagaccac cgacggcgtg gtcaaagagc aggaaatcct gggcggaggg gagagcgcca
     4561 ccgagacaag caagagcaac ctggaaaagc ccaaggacgt ggagcccagc cacgagatct
     4621 ccgagcctgt gctgtccggc accaccggca aagaggaatc cgagctgctg aagtccaaga
     4681 gcatcgagac aaagggcgag acagacccca gaagcaacga ccaggaagat gccacagacg
     4741 acgtggtgga gaacagccgg gacgacaaca atgccctgag caactccgtg gacaaccagg
     4801 ccaacgtgct gaacagagag gaccctatcg ccagcgagac agaggtggtg tccgagcctg
     4861 aggacagcag ccggatcatc accaccgagg tgcccagcac caccgtgaag ccccccgatg
     4921 agaagagatc cgaggaagtg ggcgaaaaag aagccaaaga aatcaaggtg gagcccgtgg
     4981 tgcctagagc catcggcgag cccatggaaa actccgtgag cgtgcagagc ccccccaacg
     5041 tggaggatgt ggagaaagag acactgatct ccgagaataa cggcctgcac aacgacgccc
     5101 accggggcaa tatcgccgag aaggacctga tcgacatcca tctgctgcgg aacgaggccg
     5161 gcagcacaat cctggacgac agccggcgga atggcgagat gaccgagggc agcgagtctg
     5221 atgtgggcga gctgcaggaa cataacttcg ccacccagca gaaggatgag aaggacttcg
     5281 accagatcgc ctccgaccgg gagaaagagg aaatccagaa actgctgaac atcggccacg
     5341 aagaggacga ggacgtgctg aagatggacc ggaccgagga ctccatgagc gacggcgtga
     5401 acagccacct gtactacaac aacctggcca gcgaggaaaa gatggaacag tacaacaacc
     5461 gggacgccag caaggaccgg gaagagatcc tgaaccgggc caacaccaat acctgtagca
     5521 acgagcacag cctgaagtac tgccagtaca tggaacggaa caaggatctg ctggaaacct
     5581 gtagcgagga caagcggctg cacctgtgct gcgagatcag cgactactgc ctgaagttct
     5641 tcaaccccaa gtccatcgag tacttcgact gcacccagaa agagtttgac gaccccacct
     5701 acaactgctt cagaaagcag cggttcacca gcggcgcgcc gtcgacctcc atcacggcct
     5761 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     5821 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     5881 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     5941 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     6001 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     6061 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     6121 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     6181 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     6241 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggat
     6301 cactgcatca tattctggat gcacagaaaa tggtgtggaa tcatcgttaa gtctagacac
     6361 gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat
     6421 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     6481 tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga
     6541 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     6601 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     6661 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     6721 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     6781 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     6841 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     6901 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     6961 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     7021 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     7081 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     7141 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     7201 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     7261 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     7321 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     7381 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     7441 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     7501 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     7561 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     7621 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     7681 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     7741 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     7801 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     7861 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     7921 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     7981 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     8041 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     8101 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     8161 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     8221 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     8281 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     8341 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     8401 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     8461 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     8521 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     8581 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     8641 atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata
     8701 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     8761 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8821 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8881 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     8941 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9001 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9061 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9121 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9181 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9241 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     9301 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9361 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     9421 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9481 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9541 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9601 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     9661 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9721 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9781 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     9841 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9901 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     9961 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10021 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    10081 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10141 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10201 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10261 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    10321 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    10381 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    10441 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    10501 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    10561 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    10621 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    10681 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
    10741 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
    10801 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
    10861 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
    10921 tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
    10981 tgttgccatt gctgcaggcg cagaactggt aggtatgga
//
