LOCUS       sequence-68765-e         705 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  A lentiviral vector encoding a fusion between codon-optimized 
            P.aeruginosa exoY mutant (K81M) and myc-tagged destabilized FKBP12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Ochoa CD, Alexeyev M, Pastukh V, Balczon R, Stevens T
  TITLE     Pseudomonas aeruginosa exotoxin Y is a promiscuous cyclase that 
            increases endothelial tau phosphorylation and permeability.
  JOURNAL   J Biol Chem. 2012 Jul 20;287(30):25407-18. doi: 
            10.1074/jbc.M111.301440. Epub 2012 May 25.
  PUBMED    22637478
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.301440"; journalName: "J Biol Chem"; date: 
            "2012-07-20- 20"; volume: "287"; issue: "30"; pages: "25407-18"
COMMENT     Sequence Label: pMA3228
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..343)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLGDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQGAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     ELE"
     CDS             complement(344..373)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cggccatcaa ttcctgcatt tattccagtt ccagaagctc cacatcgaag acgagagtgg
       61 catgtggtgg gatgatgcct gggtgcccag tggcaccata ggcataatct ggagatatag
      121 tcagtttggc tccctgaccc acactcatct gggcaacccc ttcttcccag cctcggatca
      181 cctcctgctt gcctagcata aacttaaagg gcttgtttct gtcccgagag gaatcgactt
      241 tctttccatc tccaagcatc ccggtgtagt gcaccacaca ggtctggccg cgcttgggga
      301 aggtgcgccc gtctcctggg gagatggttt ccacctgcac tcccaggtcc tcctcgctga
      361 tgagtttctg ctccccaacc agctccacct tcctctggaa gaagtccagg gcctcctgga
      421 agccgggcct caggggcacg ttccagtcgg ggtgggcggt gaagtggtag ccgttgccct
      481 tgatgcactc caccagcagg ctgaagctct tcctgtcggc caccacgcac acctcgtcga
      541 acctcacgct ctcctcgccc accctgtgct ccatcgccct gggcaggtag aaggtggcgg
      601 ggaagttgtc gcccatgtgg ctgccggggt tgccggcgtc gtcgctgtgg tggaacatct
      661 ccctgtgctc gcccctgccc aggcagtcgt tcagggcgtc cacca
//
