LOCUS       sequence-68805-e        8380 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8380)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 8380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB63
FEATURES             Location/Qualifiers
     source          1..8380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     38..60
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     52..69
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     53..69
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     76..95
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        76..94
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        130..729
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     CDS             753..818
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             825..845
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(870..1910,1962..2948,3000..4061,4113..5123)
                     /codon_start=1
                     /product="S. pyogenes Cas9, codon optimized for C. elegans 
                     with synthetic introns"
                     /label=Cas9
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5130..5204
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="MSRRRKANPTKLSENAKKLAKEVEN"
     terminator      5214..5537
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     promoter        complement(5645..5663)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5684..5700)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5684..5700)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5697..5719)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5708..5724
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5732..5738,5739..5756,5757..5762))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5777..5798
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5915..5932)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6086..6674)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6166..6185)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6845..7636,7637..7705))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7468..7487
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7706..7810)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7836..8291)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7973..7994)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8185..8204
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
       61 acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg gccccccctc
      121 gaggtcgacg cacctttggt cttttattgt caacttccat tggttcttcc attgtttctg
      181 ttaaattaat gaatttttca taaaataaag acattataca atataaaaat gaagaattta
      241 ttgaaaataa actgccagag agaaaaagta tgcaacactc ccgccgagag tgtttgaaat
      301 ggtgtacggt acattttcgt gctaggagtt agatgtgcag gcagcaacga gagggggaga
      361 gatttttttg ggccttgtga aattaacgtg agttttctgg tcatctgact aatcatgttg
      421 gttttttgtt ggtttatttt gtttttatct ttgtttttat ccagattagg aaatttaaat
      481 tttatgaatt tataatgagg tcaaacattc agtcccagcg tttttcctgt tctcactgtt
      541 tagtcgaatt tttattttag gctttcaaca aatgttctaa ctgtcttatt tgtgacctca
      601 ctttttatat ttttttaatt tttaaaaata ttagaagttt ctaggataat tttttcgact
      661 tttattctct ctaccgtccg cactcttctt acttttaaat taaattgttt ttttttcagt
      721 tgggaaacac tttgctcatc tagaaaaaaa tggactacaa ggaccacgac ggagactaca
      781 aggaccacga catcgactac aaggacgacg acgacaagat ggccccaaag aagaagcgta
      841 aggtcggaat ccacggagtc ccagccgccg acaagaagta ctccatcgga ctcgacatcg
      901 gaaccaactc cgtcggatgg gccgtcatca ccgacgagta caaggtccca tccaagaagt
      961 tcaaggtcct cggaaacacc gaccgtcact ccatcaagaa gaacctcatc ggagccctcc
     1021 tcttcgactc cggagagacc gccgaggcca cccgtctcaa gcgtaccgcc cgtcgtcgtt
     1081 acacccgtcg taagaaccgt atctgctacc tccaagagat cttctccaac gagatggcca
     1141 aggtcgacga ctccttcttc caccgtctcg aggagtcctt cctcgtcgag gaggacaaga
     1201 agcacgagcg tcacccaatc ttcggaaaca tcgtcgacga ggtcgcctac cacgagaagt
     1261 acccaaccat ctaccacctc cgtaagaagc tcgtcgactc caccgacaag gccgacctcc
     1321 gtctcatcta cctcgccctc gcccacatga tcaagttccg tggacacttc ctcatcgagg
     1381 gagacctcaa cccagacaac tccgacgtcg acaagctctt catccaactc gtccaaacct
     1441 acaaccaact cttcgaggag aacccaatca acgcctccgg agtcgacgcc aaggccatcc
     1501 tctccgcccg tctctccaag tcccgtcgtc tcgagaacct catcgcccaa ctcccaggag
     1561 agaagaagaa cggactcttc ggaaacctca tcgccctctc cctcggactc accccaaact
     1621 tcaagtccaa cttcgacctc gccgaggacg ccaagctcca actctccaag gacacctacg
     1681 acgacgacct cgacaacctc ctcgcccaaa tcggagacca atacgccgac ctcttcctcg
     1741 ccgccaagaa cctctccgac gccatcctcc tctccgacat cctccgtgtc aacaccgaga
     1801 tcaccaaggc cccactctcc gcctccatga tcaagcgtta cgacgagcac caccaagacc
     1861 tcaccctcct caaggccctc gtccgtcaac aactcccaga gaagtacaag gtaagtttaa
     1921 acatatatat actaactaac cctgattatt taaattttca ggagatcttc ttcgaccaat
     1981 ccaagaacgg atacgccgga tacatcgacg gaggagcctc ccaagaggag ttctacaagt
     2041 tcatcaagcc aatcctcgag aagatggacg gaaccgagga gctcctcgtc aagctcaacc
     2101 gtgaggacct cctccgtaag caacgtacct tcgacaacgg atccatccca caccaaatcc
     2161 acctcggaga gctccacgcc atcctccgtc gtcaagagga cttctaccca ttcctcaagg
     2221 acaaccgtga gaagatcgag aagatcctca ccttccgtat cccatactac gtcggaccac
     2281 tcgcccgtgg aaactcccgt ttcgcctgga tgacccgtaa gtccgaggag accatcaccc
     2341 catggaactt cgaggaggtc gtcgacaagg gagcctccgc ccaatccttc atcgagcgta
     2401 tgaccaactt cgacaagaac ctcccaaacg agaaggtcct cccaaagcac tccctcctct
     2461 acgagtactt caccgtctac aacgagctca ccaaggtcaa gtacgtcacc gagggaatgc
     2521 gtaagccagc cttcctctcc ggagagcaaa agaaggccat cgtcgacctc ctcttcaaga
     2581 ccaaccgtaa ggtcaccgtc aagcaactca aggaggacta cttcaagaag atcgagtgct
     2641 tcgactccgt cgagatctcc ggagtcgagg accgtttcaa cgcctccctc ggaacctacc
     2701 acgacctcct caagatcatc aaggacaagg acttcctcga caacgaggag aacgaggaca
     2761 tcctcgagga catcgtcctc accctcaccc tcttcgagga ccgtgagatg atcgaggagc
     2821 gtctcaagac ctacgcccac ctcttcgacg acaaggtcat gaagcaactc aagcgtcgtc
     2881 gttacaccgg atggggacgt ctctcccgta agctcatcaa cggaatccgt gacaagcaat
     2941 ccggaaaggt aagtttaaac agttcggtac taactaacca tacatattta aattttcaga
     3001 ccatcctcga cttcctcaag tccgacggat tcgccaaccg taacttcatg caactcatcc
     3061 acgacgactc cctcaccttc aaggaggaca tccaaaaggc ccaagtctcc ggacaaggag
     3121 actccctcca cgagcacatc gccaacctcg ccggatcccc agccatcaag aagggaatcc
     3181 tccaaaccgt caaggtcgtc gacgagctcg tcaaggtcat gggacgtcac aagccagaga
     3241 acatcgtcat cgagatggcc cgtgagaacc aaaccaccca aaagggacaa aagaactccc
     3301 gtgagcgtat gaagcgtatc gaggagggaa tcaaggagct cggatcccaa atcctcaagg
     3361 agcacccagt cgagaacacc caactccaaa acgagaagct ctacctctac tacctccaaa
     3421 acggacgtga catgtacgtc gaccaagagc tcgacatcaa ccgtctctcc gactacgacg
     3481 tcgaccacat cgtcccacaa tccttcctca aggacgactc catcgacaac aaggtcctca
     3541 cccgttccga caagaaccgt ggaaagtccg acaacgtccc atccgaggag gtcgtcaaga
     3601 agatgaagaa ctactggcgt caactcctca acgccaagct catcacccaa cgtaagttcg
     3661 acaacctcac caaggccgag cgtggaggac tctccgagct cgacaaggcc ggattcatca
     3721 agcgtcaact cgtcgagacc cgtcaaatca ccaagcacgt cgcccaaatc ctcgactccc
     3781 gtatgaacac caagtacgac gagaacgaca agctcatccg tgaggtcaag gtcatcaccc
     3841 tcaagtccaa gctcgtctcc gacttccgta aggacttcca attctacaag gtccgtgaga
     3901 tcaacaacta ccaccacgcc cacgacgcct acctcaacgc cgtcgtcgga accgccctca
     3961 tcaagaagta cccaaagctc gagtccgagt tcgtctacgg agactacaag gtctacgacg
     4021 tccgtaagat gatcgccaag tccgagcaag agatcggaaa ggtaagttta aacatgattt
     4081 tactaactaa ctaatctgat ttaaattttc aggccaccgc caagtacttc ttctactcca
     4141 acatcatgaa cttcttcaag accgagatca ccctcgccaa cggagagatc cgtaagcgtc
     4201 cactcatcga gaccaacgga gagaccggag agatcgtctg ggacaaggga cgtgacttcg
     4261 ccaccgtccg taaggtcctc tccatgccac aagtcaacat cgtcaagaag accgaggtcc
     4321 aaaccggagg attctccaag gagtccatcc tcccaaagcg taactccgac aagctcatcg
     4381 cccgtaagaa ggactgggac ccaaagaagt acggaggatt cgactcccca accgtcgcct
     4441 actccgtcct cgtcgtcgcc aaggtcgaga agggaaagtc caagaagctc aagtccgtca
     4501 aggagctcct cggaatcacc atcatggagc gttcctcctt cgagaagaac ccaatcgact
     4561 tcctcgaggc caagggatac aaggaggtca agaaggacct catcatcaag ctcccaaagt
     4621 actccctctt cgagctcgag aacggacgta agcgtatgct cgcctccgcc ggagagctcc
     4681 aaaagggaaa cgagctcgcc ctcccatcca agtacgtcaa cttcctctac ctcgcctccc
     4741 actacgagaa gctcaaggga tccccagagg acaacgagca aaagcaactc ttcgtcgagc
     4801 aacacaagca ctacctcgac gagatcatcg agcaaatctc cgagttctcc aagcgtgtca
     4861 tcctcgccga cgccaacctc gacaaggtcc tctccgccta caacaagcac cgtgacaagc
     4921 caatccgtga gcaagccgag aacatcatcc acctcttcac cctcaccaac ctcggagccc
     4981 cagccgcctt caagtacttc gacaccacca tcgaccgtaa gcgttacacc tccaccaagg
     5041 aggtcctcga cgccaccctc attcaccaat ccatcaccgg actctacgag acccgtatcg
     5101 acctctccca actcggagga gacctgcaga tgtcccgtcg tcgtaaggcc aacccaacca
     5161 agctctccga gaacgccaag aagctcgcca aggaggtcga gaactaggaa ttcatgcaag
     5221 atcctttcaa gcattccctt cttctctatc actcttcttt ctttttgtca aaaaattctc
     5281 tcgctaattt atttgctttt ttaatgttat tattttatga ctttttatag tcactgaaaa
     5341 gtttgcatct gagtgaagtg aatgctatca aaatgtgatt ctgtctgatg tactttcaca
     5401 atctctcttc aattccattt tgaagtgctt taaacccgaa aggttgagaa aaatgcgagc
     5461 gctcaaatat ttgtattgtg ttcgttgagt gacccaacaa aaagaggaaa ctttattgtg
     5521 ccgccaagaa aaaagtctca ttgcctaaga gaaacatatg tggttggaaa tcttccagat
     5581 cgtggatcac tagtatgtgg ttggaaatct tccagatcgt ggatcagagc tccagctttt
     5641 gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg
     5701 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     5761 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     5821 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     5881 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5941 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6001 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6061 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6121 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6181 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6241 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6301 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6361 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6421 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6481 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     6541 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6601 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6661 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6721 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6781 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6841 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6901 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6961 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7021 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7081 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7141 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7201 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7261 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7321 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7381 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7441 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7501 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7561 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7621 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7681 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7741 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7801 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     7861 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     7921 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     7981 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     8041 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     8101 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     8161 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     8221 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     8281 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     8341 ggcctcttcg ctattacgcc agctggcgaa agggggatgt
//
