LOCUS       sequence-68864-e         910 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI
            to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pINDUCER12 (miR-RUL)
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           93..186
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     186..202
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     187..203
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             224..241
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             242..265
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      275..284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gccaagtact gcgacctccc tagcaaactg gggcacagat gatgcggccg caagccttgt
       61 taagtgctcg cttcggcagc acatatacta tgttgaatga ggcttcagta ctttacagaa
      121 tcgttgcctg cacatcttgg aaacacttgc tgggattact tcttcaggtt aacccaacag
      181 aaggctcgag gtcgacggta tcgataagct tgacgtccat atgctggtac cccgtggaag
      241 tctggaagtt ctcttccagg ggcccgaagg agacgccacc atgggtctag atagggataa
      301 cagggtaatg gtaccggatc gatcccccaa cttccaggcc cgccatagtg atttgatttg
      361 ccagcgcatc gctatcaatc gccgggttca cccattcgcg taaccacagt tcactgaatt
      421 tcataatcta ttcctgcctt atttaaactg tttgaggaaa cgcagatcgt tttcgaagaa
      481 tgaacgcagg tcggtgacgc cgtaacgcaa catagtcaga cgctccatcc ccatcccgaa
      541 gccgaaacca gagtaaactt ccgggtcgat gccaacgtta cgcaacacgt tcggatgcac
      601 catcccgcag cccagcactt ccagccattt accgttttta cccatgacgt ccacttctgc
      661 agaaggttcg gtaaacggga agtaggaagg acggaagcga atctgcaaat cttcctcaaa
      721 gaagttacgc aggaagtcgt gcagcgtgcc tttcaggttg gtaaagctga tgttggtatc
      781 aacaatcaga ccttccatct gatggaacat cggcgtgtga gtctggtcgt agtcgttacg
      841 ataaacacgg ccaggcgcga tgatacgaat cggtggctgc tgggctttca tggtgcggat
      901 ctgtacgcca
//
