LOCUS       sequence-68891-e         899 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Human expression vector containing MSCV LTR promoter, dCas9 that is 
            fused to 2x NLS, p65 activation domain and tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pMSCV-LTR-dCas9-p65AD-BFP
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctcgcggat aagtttgtcg ttttcgtcgt acttggtatt catgcgtgag tcaaggattt
       61 gggctacatg tttagtaatc tgccgagtct ccacaagttg ccgtttaatg aatcctgctt
      121 tgtccagttc gctcagtccg cccctctcag ccttagtgag attgtcgaac ttccgctgag
      181 tgatcagctt tgcgttcaga agctggcgcc aatagttctt catctttttc acgacttcct
      241 ctgagggcac gttatctgac tttccccggt tcttgtctga gcgagtcagc accttattgt
      301 caattgagtc gtctttgagg aagctctgag ggacgatggc gtccacgtcg tagtctgaga
      361 gcctgttgat atcaagctct tggtccacat acatatcgcg tccattttga aggtagtaca
      421 gatagagctt ctcattctga agctgggtgt tttccaccgg gtgctcttta aggatctgtg
      481 acccgagctc cttaatccct tcctcaattc ttttcatccg ctccctgctg ttcttctgtc
      541 ccttctgggt agtctggttt tccctggcca tctcgatgac aatgttctct ggtttatggc
      601 gacccatcac cttgaccagc tcgtccacga ctttcacggt ctggagaata cccttcttaa
      661 tagcgggtga accagccaga tttgcgatat gctcgtgcag tgagtcacct tgtccagaca
      721 cttgggcctt ctggatgtcc tccttaaagg tcagggaatc atcatggata agttgcatga
      781 agttgcgatt agcgaatccg tcgctcttaa ggaaatcaag aatggtcttt ccgctctgtt
      841 tgtcccggat tccgttgatg agtttgcggc taaggcgtcc ccatccggta tatcttctc
//
