LOCUS       sequence-69184-e         865 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 
            (N-terminal tag). Confers resistance to G418..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Britton S, Coates J, Jackson SP
  TITLE     A new method for high-resolution imaging of Ku foci to decipher 
            mechanisms of DNA double-strand break repair.
  JOURNAL   J Cell Biol. 2013 Jul 29.
  PUBMED    23897892
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2013 Jul 29."
COMMENT     Sequence Label: pEGFP-C1-FLAG-Ku70
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..51
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctgtaca agtccggact cagatctgat tacaaggatg acgacgataa gctcgagtca
       61 gggtgggagt catattacaa aaccgagggc gatgaagaag cagaggaaga acaagaagag
      121 aaccttgaag caagtggaga ctataaatat tcaggaagag atagtttgat ttttttggtt
      181 gatgcctcca aggctatgtt tgaatctcag agtgaagatg agttgacacc ttttgacatg
      241 agcatccagt gtatccaaag tgtgtacatc agtaagatca taagcagtga tcgagatctc
      301 ttggctgtgg tgttctatgg taccgagaaa gacaaaaatt cagtgaattt taaaaatatt
      361 tacgtcttac aggagctgga taatccaggt gcaaaacgaa ttctagagct tgaccagttt
      421 aaggggcagc agggacaaaa acgtttccaa gacatgatgg gccacggatc tgactactca
      481 ctcagtgaag tgctgtgggt ctgtgccaac ctctttagtg atgtccaatt caagatgagt
      541 cataagagga tcatgctgtt caccaatgaa gacaaccccc atggcaatga cagtgccaaa
      601 gccagccggg ccaggaccaa agccggtgat ctccgagata caggcatctt ccttgacttg
      661 atgcacctga agaaacctgg gggctttgac atatccttgt tctacagaga tatcatcagc
      721 atagcagagg atgaggacct cagggttcac tttgaggaat ccagcaagct agaagacctg
      781 ttgcggaagg ttcgcgccaa ggagacaagg aagcgagcac tcagcaggtt aaagctgaag
      841 ctcaacaaag atatagtgat ctctg
//
