LOCUS       sequence-69345-e         933 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses mutant (R256C) human MAP3K5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  TITLE     MAP3K5 expression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1-MAP3K5 (R256C)
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        58..162
                     /gene="bla"
                     /label=AmpR promoter
     CDS             347..725
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(381..400)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gggatcggga gatctcccga tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc
       61 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      121 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      181 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      241 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      301 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
      361 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
      421 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      481 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      541 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      601 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      661 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      721 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      781 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      841 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      901 ctggggccag atggtaagcc ctcccgtatc gta
//
