LOCUS       sequence-69560-e         814 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Bennett RD, Mauer AS, Strehler EE
  TITLE     Calmodulin-like protein increases filopodia-dependent cell motility 
            via up-regulation of myosin-10.
  JOURNAL   J Biol Chem. 2007 Feb 2;282(5):3205-12. Epub 2006 Nov 26.
  PUBMED    17130134
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-02-2"; volume: "282(5)"; pages: "3205-12. Epub 
            2006 Nov 26"
COMMENT     Sequence Label: EGFPC1-hMyoX
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..44
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..80
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      344..353
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      468..477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agtccggact cagatcctac ccctatgatg tgcctgacta tgcccaccac caccaccacc
       61 acctggtgcc caggggcagc agatctcgag ctcaagcttc gaattctgca gtcgacggta
      121 ccatggataa cttcttcacc gagggaacac gggtctggct gagagaaaat ggccagcatt
      181 ttccaagtac tgtaaattcc tgtgcagaag gcatcgtcgt cttccggaca gactatggtc
      241 aggtattcac ttacaagcag agcacaatta cccaccagaa ggtgactgct atgcacccca
      301 cgaacgagga gggcgtggat gacatggcgt ccttgacaga gctccatggc ggctccatca
      361 tgtataactt attccagcgg tataagagaa atcaaatata tacctacatc ggctccatcc
      421 tggcctccgt gaacccctac cagcccatcg ccgggctgta cgagcctgcc accatggagc
      481 agtacagccg gcgccacctg ggcgagctgc ccccgcacat cttcgccatc gccaacgagt
      541 gctaccgctg cctgtggaag cgctacgaca accagtgcat cctcatcagt ggtgaaagtg
      601 gggcaggtaa aaccgaaagc actaaattga tcctcaagtt tctgtcagtc atcagtcaac
      661 agtctttgga attgtcctta aaggagaaga catcctgtgt tgaacgagct attcttgaaa
      721 gcagccccat catggaagct ttcggcaatg cgaagaccgt gtacaacaac aactctagtc
      781 gctttgggaa gtttgttcag ctgaacatct gtca
//
