LOCUS       sequence-6976-ex       10450 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Weigel D, Ahn JH, Blazquez MA, Borevitz JO, Christensen SK, 
            Fankhauser C, Ferrandiz C, Kardailsky I, Malancharuvil EJ, Neff MM, 
            Nguyen JT, Sato S, Wang ZY, Xia Y, Dixon RA, Harrison MJ, Lamb CJ, 
            Yanofsky MF, Chory J
  TITLE     Activation tagging in Arabidopsis.
  JOURNAL   Plant Physiol. 2000 Apr . 122(4):1003-13.
  PUBMED    10759496
REFERENCE   2  (bases 1 to 10450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2000 Apr . 122(4):1003-13."
COMMENT     Sequence Label: pSKI074
FEATURES             Location/Qualifiers
     source          1..10450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(13..31)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(52..68)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(52..68)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(65..87)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    76..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(100..106,107..124,125..130))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    145..166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(283..300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(454..1042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(534..553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1213..2004,2005..2073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1836..1855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2074..2178)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2207..2662)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2344..2365)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2556..2575
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2792..2814
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2806..2823
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2807..2823
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2830..2849
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2830..2848
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2881..2897
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2881..2897
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    4270..4294
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     oriT            5497..5606
                     /note="incP origin of transfer"
     rep_origin      6401..7113
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    7700..7724
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     primer_bind     complement(8045..8061)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8046..8062)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        8362..8741
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             8771..9565
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8825..8844)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9435..9454
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      9761..10011
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
ORIGIN
        1 cagcttttgt tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct
       61 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      121 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      181 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      241 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
      901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1801 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2161 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggaaat tgtaaacgtt
     2221 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     2281 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     2341 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     2401 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     2461 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     2521 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     2581 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     2641 aatgcgccgc tacagggcgc gtcgcgccat tcgccattca ggctgcgcaa ctgttgggaa
     2701 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     2761 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     2821 agtgaattgt aatacgactc actatagggc gaattggagc tccaccgcgg tggcggccgc
     2881 tctagaacta gtggatcccc aacatggtgg agcacgacac tctcgtctac tccaagaata
     2941 tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat
     3001 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
     3061 aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
     3121 atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa
     3181 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tagatcccca
     3241 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     3301 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
     3361 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca
     3421 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc
     3481 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
     3541 cttcaaagca agtggattga tgtgatatct agatccccaa catggtggag cacgacactc
     3601 tcgtctactc caagaatatc aaagatacag tctcagaaga ccagagggct attgagactt
     3661 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
     3721 tcatcgaaag gacagtagaa aaggaagatg gcttctacaa atgccatcat tgcgataaag
     3781 gaaaggctat cgttcaagat gcctctaccg acagtggtcc caaagatgga cccccaccca
     3841 cgaggaacat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
     3901 gtgatatcta gatccccaac atggtggagc acgacactct cgtctactcc aagaatatca
     3961 aagatacagt ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg
     4021 gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa
     4081 aggaagatgg cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
     4141 cctctaccga cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag
     4201 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctag atccgaaact
     4261 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     4321 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     4381 ccaaccacag ggttcccctc gggagtgctt ggcattccgt gcgataatga cttctgttca
     4441 accacccaaa cgtcggaaag cctgacgacg gagcagcatt ccaaaaagat cccttggctc
     4501 gtctgggtcg gctagaaggt cgagtgggct gctgtggctt gatccctcaa cgcggtcgcg
     4561 gacgtagcgc agcgccgaaa aatcctcgat cgcaaatccg acgctgtcga aaagcgtgat
     4621 ctgcttgtcg ctctttcggc cgacgtcctg gccagtcatc acgcgccaaa gttccgtcac
     4681 aggatgatct ggcgcgagtt gctggatctc gccttcaatc cgggtctgtg gcgggaactc
     4741 cacgaaaata tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc ctgggcagtc
     4801 gccgccgacg ccgttgatgt ggacgccggg cccgatcata ttgtcgctca ggatcgtggc
     4861 gttgtgcttg tcggccgttg ctgtcgtaat gatatcggca ccttcgaccg cctgttccgc
     4921 agaggtgcag gcctcgatct gaaacccgaa ccgctggaga ttgcgggagc agcgagcagt
     4981 agcctcgggg tcgatgtcgt aaagtcgtat ccgatcgacg ccgatcagcg ccttgaaggc
     5041 caaagcctgg aactcacttt gggcaccgtt gccgatcagc gccatcgtgc gcgaatcttt
     5101 acgggccaga tactttgccg cgatcgcgga ggtcgcggcc gttcgcaagg ccgtcaggat
     5161 tgtcatttcc gacagcagca gcggatagcc gctatcgaca tcggagagca cgccgaacgc
     5221 ggttaccggc ctcttcatcg ggaatgcgcg cgaccttcag catcgccggc atgtccccct
     5281 ggcggacggg aagtatccag ctcgagatcc ggattttgta gccctggccg acggccagca
     5341 ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt
     5401 ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg gtttcatcag
     5461 ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat
     5521 tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg
     5581 gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta
     5641 cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa
     5701 tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgctgcttc cctgctgttt
     5761 tgtggaatat ctaccgactg gaaacaggca aatgcaggaa attactgaac tgaggggaca
     5821 ggcgagagac gatgccaaag agctacaccg acgagctggc cgagtgggtt gaatcccgcg
     5881 cggccaagaa gcgccggcgt gatgaggctg cggttgcgtt cctggcggtg agggcggatg
     5941 tcgaggcggc gttagcgtcc ggctatgcgc tcgtcaccat ttgggagcac atgcgggaaa
     6001 cggggaaggt caagttctcc tacgagacgt tccgctcgca cgccaggcgg cacatcatan
     6061 gccaagcccg ccgatgtgcc cgcaccgcag gccaaggctg cggaacccgc gccggcaccc
     6121 aagacgccgg agccacggcg gccgaagcag gggggcaagg ctgaaaagcc ggcccccgct
     6181 gcggccccga ccggcttcac nttcaaccca acaccggaca aaaaggatct agcgtggact
     6241 caaggctctc gcgaatggct cgcgttggaa actttcattg acacttgagg ggcaccgcag
     6301 ggaaattctn gtccttgcga gaaccggcta tgtcgtgctg cgcatcgagc ctgcgccctt
     6361 ggcttgtctc gcccctctcc gcgtcgctac ggggcttcca gcgcctttcc gacgctcacc
     6421 gggctggttg ccctcgccgc tgggctggcg gccgttctat ggccctgcaa acgcgccaga
     6481 aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg cgttgnggat acctngcgga
     6541 aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc
     6601 cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc
     6661 agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac
     6721 aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg
     6781 aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt
     6841 gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt
     6901 tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt
     6961 ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc
     7021 ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg ctgcgcccct
     7081 cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc agtccttgnc nnngcnngga
     7141 tcggggcnnt nnnggnatgg gcgatcagcc cgagcgcgac gcccggaagc attgacgtgc
     7201 cgcaggtgct ggcatcnaca ttcagcgacc aggtgccggg cagtgagggc ggcggcctgg
     7261 gtggcggcct gcccttcact tcggccgtcg gggcattcac ggacttcatg gcggggccgg
     7321 caatttttac cttgggcatt cttggcatan tggtcgcggg tgccgtgctc gtgttcgggg
     7381 gtgaattgca agctagcttg cttggtcgtt ccggtaccgt gaacgtcggc tcgattgtac
     7441 ctgcgttcaa atactttgcg atcgtgttgc gcgcctgccc ggtgcgtcgg ctgatctcac
     7501 ggatcgactg cttctctcgc aacgccatcc gacggatgat gtttaaaagt cccatgtgga
     7561 tcactccgtt gccccgtcgc tcaccgtgtt ggggggaagg tgcacatggc tcagttctca
     7621 atggaaatta tctgcctaac cggctcagtt ctgcgtagaa accaacatgc aagctccacc
     7681 gggtgcaaag cggcagcggc ggcaggatat attcaattgt aaatggcttc atgtccggga
     7741 aatctacatg gatcagcaat gagtatgatg gtcaatatgg aggaaaagaa agagtaatta
     7801 ccaatttttt ttcaattcaa aaatgtagat gtccgcagcg ttattataaa atgaaagtac
     7861 attttgataa aacgacaaat tacgatccgt cgtatttata ggcgaaagca ataaacaaat
     7921 tattctaatt cggaaatctt tatttcgacg tgtctacatt cacgtccaaa tgggggctta
     7981 gatgagaaac ttcacgatcg atatctagat ctcgagctcg agatctagat atcgataagc
     8041 ttatcgatac cgtcgacctc gaggggccaa cagagcctgg cgttcccctt ttgcattgag
     8101 accgatgttc gttccggaac cttgcacgcc ccagagcttc tcaccgttca cgacaatttc
     8161 cttctcgttg aggtcggtcg cgccatgtcg gatgaaataa aaacttttga taccagcggg
     8221 ggccttcgca gagccgaggt aggtctgaga aattggcatt ttcacgtgtg gaagatatga
     8281 atttttttga gaaactagat aagattaatg aatatcggtg ttttggtttt ttcttgtggc
     8341 cgtctttgtt tatattgaga tttttcaaat cagtgcgcaa gacgtgacgt aagtatctga
     8401 gctagttttt atttttctac taatttggtc gtttatttcg gcgtgtagga catggcaacc
     8461 gggcctgaat ttcgcgggta ttctgtttct attccaactt tttcttgatc cgcagccatt
     8521 aacgactttt gaatagatac gctgacacgc caagcctcgc tagtcaaaag tgtaccaaac
     8581 aacgctttac agcaagaacg gaatgcgcgt gacgctcgcg gtgacgccat ttcgcctttt
     8641 cagaaatgga taaatagcct tgcttcctat tatatcttcc caaataccaa tacattacac
     8701 tagcatctga atttcataac caatctcgat acaccaaatc gactctagcg aattccccgg
     8761 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     8821 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     8881 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     8941 gaatgaactc caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     9001 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     9061 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     9121 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     9181 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     9241 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     9301 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     9361 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     9421 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     9481 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     9541 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     9601 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     9661 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     9721 gagttcttcg cccaccccgg aattccagct tccctgaaac cttggactcc catgttggca
     9781 aaggcaacca aacaaacaag aagatccgct cctgcatatg gggcggtttg agtatttcaa
     9841 ctgccatttg ggctgaattg aagacatgct cctgtcagaa attccgtgat cttactcaat
     9901 attcagtaat ctcggccaat atcctaaatg tgcgtggctt tatctgtctt tgtattgttt
     9961 catcaattca tgtaacgttt gcttttctta tgaattttca aataaattat cgatagtact
    10021 acgaatattt cgtatcgctg atcttctcaa tcacaatgat gcgtagtgac ccgacaaata
    10081 atttaagcgt ccttaatacc aatcctaaaa taattgaggc aaataaaatt tttttgtaat
    10141 ttttatgata gcagatcgat tctccagcaa gcctgcaaca aaatattgtg tatttctaaa
    10201 tagattttga tattaaaatc ccgagaaagc aaaattgcat ttaacaaaac agtaatttag
    10261 tacattaata aaaattatgc tcaaacattt ccatgaaatt aaaacataat tacattttac
    10321 aacacaatat tttgcgatat tattgccttt cgccaattta acactggaag caattaaagt
    10381 aggcccacac agagcgcttt gcaacactga caacgcttgc ggttggtcgg ctcgaggggg
    10441 ggcccggtac
//
