LOCUS       sequence-70477-e         988 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  BIOFAB reporter plasmid for measuring rrnB T1 termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB808
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             23..34
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(124..195)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(285..962)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             969..980
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cttctccttt gctcatggta cctttctcct ctttaatgaa ttgattaacc ggaagaaggt
       61 caatcataaa ggccactctt gcgaatgacc ttgagtttgt ccctctatag tgagtccgcc
      121 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc
      181 ctttcgtttt atttgatgcc tggcagttcc catcacataa gtaaagtgat aatcataaag
      241 gccactcgct aggagcgacc ttgagtctat cactcagcag attattaagc accggtggag
      301 tgacgacctt cagcacgttc gtactgttca acgatggtgt agtcttcgtt gtgggaggtg
      361 atgtccagtt tgatgtcggt tttgtaagca cccggcagct gaaccggttt tttagccatg
      421 taggtggttt taacttcagc gtcgtagtga ccaccgtctt tcagtttcag acgcattttg
      481 atttcacctt tcagagcacc gtcttccggg tacatacgtt cggtggaagc ttcccaaccc
      541 atggtttttt tctgcataac cggaccgtcg gacgggaagt tggtaccacg cagtttaact
      601 ttgtagatga actcaccgtc ttgcagggag gagtcctggg taacggtaac aacaccaccg
      661 tcttcgaagt tcataacacg ttcccatttg aaaccttccg ggaaggacag tttcaggtag
      721 tccgggatgt cagccgggtg tttaacgtaa gctttggaac cgtactggaa ctgcggggac
      781 aggatgtccc aagcgaacgg cagcggacca cctttggtaa ctttcagttt agcggtctgg
      841 gtaccttcgt acggacgacc ttcaccttca ccttcgattt cgaactcgtg accgttaacg
      901 gaaccttcca tacgaacttt gaaacgcatg aactctttga taacgtcttc gctactcgcc
      961 atggtacctt tctcctcttt aatgaatt
//
