LOCUS       sequence-7049-ex        8909 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006  . 6():13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol. 2006  . 6():13."
COMMENT     Sequence Label: V623 pAcGHLT-LP - Acceptor
FEATURES             Location/Qualifiers
     source          1..8909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     1269..2096
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             1269..1393
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(1431..2036)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        2100..2191
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2116..2133
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             2206..2859
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     2817..2839
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             2875..2892
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2926..2943
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(2965..2998)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_recomb     3201..4659
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           3289..3661
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(3318..4659)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(5803..5820)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5803..5819)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5812..5834)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6032..6487
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6119..6138)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6329..6350
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6504..6523)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6623..6645
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6683..6701)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6769..6873
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6874..6942,6943..7734)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7092..7111)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7905..8493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8394..8413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8647..8664
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8781..8802
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8817..8822,8823..8840,8841..8847)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8855..8871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8860..8882
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8879..8895
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8879..8895
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
       61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
      121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
      181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
      241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
      301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
      361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
      421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
      481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
      541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
      601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
      661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
      721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
      781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
      841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
      901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
      961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
     1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
     1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
     1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
     1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
     1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
     1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
     1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
     1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
     1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
     1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
     1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
     1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
     1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
     1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
     1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
     1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
     2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
     2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatacta
     2221 ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa
     2281 gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag
     2341 tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta
     2401 acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt
     2461 ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt
     2521 gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag
     2581 ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt
     2641 gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg
     2701 gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct
     2761 atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc
     2821 tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatccgat gggacatcat
     2881 catcatcatc acggaaggag aagggccagt gttgcggcgg gaattttggt ccctcgtgga
     2941 agcccaggac tcgatggcat atggataact tcgtatagca tacattatac gaagttatag
     3001 atccaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3061 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3121 gacgtgaatt caggcctcca tgggagctcg cggccgcctg cagggtaccc ccgggagatc
     3181 tgtaccgact ctgctgaaga ggaggaaatt ctccttgaag tttccctggt gttcaaagta
     3241 aaggagtttg caccagacgc acctctgttc actggtccgg cgtattaaaa cacgatacat
     3301 tgttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac agacaattgt
     3361 tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt tagagcgaaa
     3421 atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc aatagatttg
     3481 taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat ggctggacta
     3541 tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag caatctagct
     3601 ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt caaaatatta
     3661 tgcgcttttg tatttctttc atcactgtcg ttagtgtaca attgactcga cgtaaacacg
     3721 ttaaataaag cttggacata tttaacatcg ggcgtgttag ctttattagg ccgattatcg
     3781 tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag acgattttgc catagccaca
     3841 cgacgcctat taattgtgtc ggctaacacg tccgcgatca aatttgtagt tgagcttttt
     3901 ggaattattt ctgattgcgg gcgtttttgg gcgggtttca atctaactgt gcccgatttt
     3961 aattcagaca acacgttaga aagcgatggt gcaggcggtg gtaacatttc agacggcaaa
     4021 tctactaatg gcggcggtgg tggagctgat gataaatcta ccatcggtgg aggcgcaggc
     4081 ggggctggcg gcggaggcgg aggcggaggt ggtggcggtg atgcagacgg cggtttaggc
     4141 tcaaatgtct ctttaggcaa cacagtcggc acctcaacta ttgtactggt ttcgggcgcc
     4201 gtttttggtt tgaccggtct gagacgagtg cgattttttt cgtttctaat agcttccaac
     4261 aattgttgtc tgtcgtctaa aggtgcagcg ggttgaggtt ccgtcggcat tggtggagcg
     4321 ggcggcaatt cagacatcga tggtggtggt ggtggtggag gcgctggaat gttaggcacg
     4381 ggagaaggtg gtggcggcgg tgccgccggt ataatttgtt ctggtttagt ttgttcgcgc
     4441 acgattgtgg gcaccggcgc aggcgccgct ggctgcacaa cggaaggtcg tctgcttcga
     4501 ggcagcgctt ggggtggtgg caattcaata ttataattgg aatacaaatc gtaaaaatct
     4561 gctataagca ttgtaatttc gctatcgttt accgtgccga tatttaacaa ccgctcaatg
     4621 taagcaattg tattgtaaag agattgtctc aagctcggat cgatcccgca cgccgataac
     4681 aagccttttc atttttacta cagcattgta gtggcgagac acttcgctgt cgtcgacgta
     4741 catgtatgct ttgttgtcaa aaacgtcgtt ggcaagcttt aaaatattta aaagaacatc
     4801 tctgttcagc accactgtgt tgtcgtaaat gttgtttttg ataatttgcg cttccgcagt
     4861 atcgacacgt tcaaaaaatt gatgcgcatc aattttgttg ttcctattat tgaataaata
     4921 agattgtaca gattcatatc tacgattcgt catggccacc acaaatgcta cgctgcaaac
     4981 gctggtacaa ttttacgaaa actgcaaaaa cgtcaaaact cggtataaaa taatcaacgg
     5041 gcgctttggc aaaatatcta ttttatcgca caagcccact agcaaattgt atttgcagaa
     5101 aacaatttcg gcgcacaatt ttaacgctga cgaaataaaa gttcaccagt taatgagcga
     5161 ccacccaaat tttataaaaa tctattttaa tcacggttcc atcaacaacc aagtgatcgt
     5221 gatggactac attgactgtc ccgatttatt tgaaacacta caaattaaag gcgagctttc
     5281 gtaccaactt gttagcaata ttattagaca gctgtgtgaa gcgctcaacg atttgcacaa
     5341 gcacaatttc atacacaacg acataaaact cgaaaatgtc ttatatttcg aagcacttga
     5401 tcgcgtgtat gtttgcgatt acggattgtg caaacacgaa aactcactta gcgtgcacga
     5461 cggcacgttg gagtatttta gtccggaaaa aattcgacac acaactatgc acgtttcgtt
     5521 tgactggtac gcggcgtgtt aacatacaag ttgctaaccg gcggccgaca cccatttgaa
     5581 aaaagcgaag acgaaatgtt ggacttgaat agcatgaagc gtcgtcagca atacaatgac
     5641 attggcgttt taaaacacgt tcgtaacgtt aacgctcgtg actttgtgta ctgcctaaca
     5701 agatacaaca tagattgtag actcacaaat tacaaacaaa ttataaaaca tgagtttttg
     5761 tcgtaaaaat gccacttgtt ttacgagtag aattagcttg gcactggccg tcgttttaca
     5821 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     5881 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     5941 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     6001 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     6061 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6121 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6181 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6241 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6301 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6361 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6421 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     6481 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     6541 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     6601 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     6661 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     6721 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6781 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6841 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6901 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6961 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7021 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7081 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7141 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7201 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7261 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7321 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7381 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7441 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7501 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7561 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7621 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7681 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7741 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7801 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7861 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7921 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7981 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8041 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8101 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8161 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8221 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8281 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8341 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8401 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8461 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8521 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8581 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8641 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8701 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     8761 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8821 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     8881 ggaaacagct atgaccatga ttacgaatt
//
