LOCUS       sequence-7070-ex        1000 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Stennicke HR, Salvesen GS
  TITLE     Biochemical characteristics of caspases-3, -6, -7, and -8.
  JOURNAL   J Biol Chem. 1997 Oct 10. 272(41):25719-23.
  PUBMED    9325297
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1997 Oct 10. 272(41):25719-23."
COMMENT     Sequence Label: pcDNA3-Casp7-Flag
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             922..945
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(976..994)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(977..994)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 aagcttggta ccatggcaga tgatcagggc tgtattgaag agcagggggt tgaggattca
       61 gcaaatgaag attcagtgga tgctaagcca gaccggtcct cgtttgtacc gtccctcttc
      121 agtaagaaga agaaaaatgt caccatgcga tccatcaaga ccacccggga ccgagtgcct
      181 acatatcagt acaacatgaa ttttgaaaag ctgggcaaat gcatcataat aaacaacaag
      241 aactttgata aagtgacagg tatgggcgtt cgaaacggaa cagacaaaga tgccgaggcg
      301 ctcttcaagt gcttccgaag cctgggtttt gacgtgattg tctataatga ctgctcttgt
      361 gccaagatgc aagatctgct taaaaaagct tctgaagagg accatacaaa tgccgcctgc
      421 ttcgcctgca tcctcttaag ccatggagaa gaaaatgtaa tttatgggaa agatggtgtc
      481 acaccaataa aggatttgac agcccacttt aggggggata gatgcaaaac ccttttagag
      541 aaacccaaac tcttcttcat tcaggcttgc cgagggaccg agcttgatga tggcatccag
      601 gccgactcgg ggcccatcaa tgacacagat gctaatcctc gatacaagat cccagtggaa
      661 gctgacttcc tcttcgccta ttccacggtt ccaggctatt actcgtggag gagcccagga
      721 agaggctcct ggtttgtgca agccctctgc tccatcctgg aggagcacgg aaaagacctg
      781 gaaatcatgc agatcctcac cagggtgaat gacagagttg ccaggcactt tgagtctcag
      841 tctgatgacc cacacttcca tgagaagaag cagatcccct gtgtggtctc catgctcacc
      901 aaggaactct acttcagtca agactacaag gacgacgatg acaagtagct cgagcatgca
      961 tctagagggc cctattctat agtgtcacct aaatgctaga
//
