LOCUS       sequence-70732-e         826 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses myc tagged Miro1 E208K/E328K mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Fransson S, Ruusala A, Aspenstrom P
  TITLE     The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in 
            mitochondrial trafficking.
  JOURNAL   Biochem Biophys Res Commun. 2006 Jun 2;344(2):500-10. Epub 2006 Apr 
            3.
  PUBMED    16630562
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun."; date: "2006-06-2"; volume: "344(2)"; pages: 
            "500-10. Epub 2006 Apr 3"
COMMENT     Sequence Label: pRK5-myc-Miro1 E208K/E328K
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..68
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 actgcacctc ggttctatcg attgaattgg ccaccatgga gcagaagctg atctccgagg
       61 aggacctggg atctatgaag aaagacgtgc ggatcctgct ggtgggagaa cctagagttg
      121 ggaagacatc actgattatg tctctggtca gtgaagaatt tccagaagag gttcctcccc
      181 gggcagaaga aatcaccatt ccagctgatg tcaccccaga gagagttcca acacacattg
      241 tagattactc agaagcagaa cagagtgatg aacaacttca tcaagaaata tctcaggcta
      301 atgtcatctg tatagtgtat gccgttaaca acaagcattc tattgataag gtaacaagtc
      361 gatggattcc tctcataaat gaaagaacag acaaagacag caggctgcct ttaatattgg
      421 ttgggaacaa atctgatctg gtggaatata gtagtatgga gaccatcctt cctattatga
      481 accagtatac agaaatagaa acctgtgtgg agtgttcagc gaaaaacctg aagaacatat
      541 cagagctctt ttattacgca cagaaagctg ttcttcatcc tacagggccc ctgtactgcc
      601 cagaggagaa ggagatgaaa ccagcttgta taaaagccct tactcgtata tttaaaatat
      661 ctgatcaaga taatgatggt actctcaatg atgctaaact caacttcttt cagaggattt
      721 gtttcaacac tccattagct cctcaagctc tggaggatgt caagaatgta gtcagaaaac
      781 atataagtga tggtgtggct gacagtgggt tgaccctgaa aggttt
//
