LOCUS       sequence-70792-e         772 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Aranko AS, Oeemig JS, Kajander T, Iwai H
  TITLE     Intermolecular domain swapping induces intein-mediated protein 
            alternative splicing.
  JOURNAL   Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320.
  PUBMED    23974115
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320."
COMMENT     Sequence Label: pSABAD331
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             421..438
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(478..495)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      648..734
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc tagaaataat
       61 tttgtttaac tttaagaagg agatatacat atggaagttt atacgtcact tgaagcgaca
      121 ttcgatagag taaagtctat agcgtacgag aagggtgatt ttgatgtata cgatctttcc
      181 gtaccagaat acggcaggaa ctttataggc ggagaaggac ttctcgtact tcacaacacg
      241 gttataggta cccagtacaa acttatcctg aacggtaaaa ccctgaaagg tgaaaccacc
      301 accgaagctg ttgacgctgc taccgcggaa aaagttttca aacagtacgc taacgacaac
      361 ggtgttgacg gtgaatggac ctacgacgac gctaccaaaa cctacaccgt aacggaattc
      421 catcatcatc atcatcatta agcttggctg ttttggcgga tgagagaaga ttttcagcct
      481 gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag
      541 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
      601 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa
      661 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
      721 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cc
//
