LOCUS       sequence-71145-e         944 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length EBA175 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: EBA175-bio
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     494..513
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      536..545
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gaaccgtcag atcctcactc tcttccgcat cgctgtctgc gagggccagc tgttgggctc
       61 gcggttgagg acaaactctt cgcggtcttt ccagtactct tggatcggaa acccgtcggc
      121 ctccgaacgg tactccgcca ccgagggacc tgagcgagtc cgcatcgacc ggatcggaaa
      181 acctctcgag aaaggcgtct aaccagtcac agtcgcaagg taggctgagc accgtggcgg
      241 gcggcagcgg gtggcggtcg gggttgtttc tggcggaggt gctgctgatg atgtaattaa
      301 agtaggcggt cttgagacgg cggatggtcg aggtgaggtg tggcaggctt gagatccagc
      361 tgttggggtg agtactccct ctcaaaagcg ggcattactt ctgcgctaag attgtcagtt
      421 tccaaaaacg aggaggattt gatattcacc tggcccgatc tggccataca cttgagtgac
      481 aatgacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca agtttgccac
      541 catggagttt cagacccagg tactcatgtc cctgctgctc tgcatgtctg gtgcggccgc
      601 cagaaacgag tacgacatca aagagaacga gaagttcctg gacgtgtaca aagagaagtt
      661 caacgagctg gacaagaaaa agtacggcaa cgtgcagaaa accgacaaga agatcttcac
      721 cttcatcgag aacaagctgg acatcctgaa caacgccaag ttcaacaagc ggtggaagtc
      781 ctacggcacc cccgacaaca tcgacaagaa catggccctg atcaacaagc acaacaacga
      841 ggaaatgttc aacaacaact accagagctt cctgagcacc agcagcctga tcaagcagaa
      901 taagtacgtg cccatcaacg ccgtgcgggt gtccagaatc ctga
//
