LOCUS       sequence-71307-e        1654 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Empty destination vector for creating a GreenGate plant 
            transformation plasmid with the plant resistance cassette at the 
            T-DNA left border..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGZ003
FEATURES             Location/Qualifiers
     source          1..1654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    6..28
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             215..874
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(281..300)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             893..1198
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1077..1096
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(1228..1250)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1444..1463)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    1605..1629
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gatcttggca ggatatattg tggtgtaacg ttggatctgg ctgagaacgc cagctgtgca
       61 tgcttggtct agaatactga gaccctcgag gcttactaaa agccagataa cagtatgcgt
      121 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
      181 caaaaagagg tgtgctaagc ttaggaggtc agctatggag aaaaaaatca ctggatatac
      241 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
      301 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
      361 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
      421 tcatccggag ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
      481 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
      541 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
      601 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
      661 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
      721 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
      781 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
      841 acagtactgc gatgagtggc agggcggggc gtaagagctc aggaggacag ctatgcagtt
      901 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
      961 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     1021 agataaagtc tcccgtgaac tttacccagt ggtgcatatc ggggatgaaa gctggcgcat
     1081 gatgaccacc gatatggcca gtgtgccagt ctccgttatc ggggaagaag tggctgatct
     1141 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaac
     1201 tagtaggagg taatcaatgc tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     1261 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     1321 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
     1381 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     1441 cagtacaatc tgctctgatg ccgcatgatc atctggcgga cctcttcgga ggtccgcttt
     1501 tttttgaatt cggtctcaag gtggtacctg gtgcgatcgc tgttggcgcg ccgtgtttaa
     1561 ttaaggttgc ggccgcttac ttcgtccgag cctagttcga gccttgacag gatatattgg
     1621 cgggtaaact aagtcgctgt atgtgtttgt ttga
//
