LOCUS       sequence-71627-e        1100 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  TITLE     Series-9: N- or C-terminal Tag E.coli T7 Expression Vectors with 
            Biobrick Polycistronic Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 TEV cloning vector with BioBrick
            poly...
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             159..181
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             201..218
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             219..239
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(354..372)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      368..415
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(781..786,787..803,804..809))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(836..854)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        922..1026
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
       61 aattaatacg actcactata gggagaccac aacggtttcc ctctagtgcc ggctccggag
      121 agctctttaa tgcggccgct taattaacta gaaataattt tgtttaactt taagaaggag
      181 atatacatat gaaatcttct caccatcacc atcaccatga aaacctgtac ttccaatcca
      241 atattggaag tggataacgg atcgcgatcg ccctgcaggc cggccggtac cacgcgtgcg
      301 cgctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      361 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      421 aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga
      481 tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg
      541 ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat
      601 agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca
      661 taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat
      721 tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc
      781 attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc
      841 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
      901 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      961 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataaca ttgaaaaagg
     1021 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     1081 cttcctgttt ttgctcaccc
//
