LOCUS       sequence-71652-e        1111 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1111)
  TITLE     Series-9: N- or C-terminal Tag E.coli T7 Expression Vectors with 
            Biobrick Polycistronic Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET StrepII TEV cloning vector with BioBrick
            p...
FEATURES             Location/Qualifiers
     source          1..1111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             159..181
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             198..221
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             228..248
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(363..381)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      377..424
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(790..795,796..812,813..818))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(845..863)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        931..1035
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
       61 aattaatacg actcactata gggagaccac aacggtttcc ctctagtgcc ggctccggag
      121 agctctttaa tgcggccgct taattaacta gaaataattt tgtttaactt taagaaggag
      181 atatacatat ggctagctgg agccacccgc agttcgaaaa aggcgccgaa aacctgtact
      241 tccaatccaa tattggaagt ggataacgga tcgcgatcgc cctgcaggcc ggccggtacc
      301 acgcgtgcgc gctgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      361 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      421 tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg
      481 cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt
      541 cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca
      601 gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca
      661 gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga
      721 tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata
      781 aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga
      841 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      901 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      961 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataacat
     1021 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1081 gcattttgcc ttcctgtttt tgctcaccca g
//
