LOCUS       sequence-72297-e         470 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  TALE-mRuby2 against telomere sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Miyanari Y, Ziegler-Birling C, Torres-Padilla ME
  TITLE     Live visualization of chromatin dynamics with fluorescent TALEs.
  JOURNAL   Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680.
  PUBMED    24096363
REFERENCE   2  (bases 1 to 470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680."
COMMENT     Sequence Label: pTALYM16-Telo1
FEATURES             Location/Qualifiers
     source          1..470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           complement(272..314)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     primer_bind     complement(338..357)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(339..357)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(424..446)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 attatgtgct gatacgtgac agcgacggtc cctaacgctg ccgggtgttg gctgagcgca
       61 acgatgtgcg cgtgtgtaaa cccatggccc accagtgcct cgtggtgctg cgccactgtc
      121 gaacgcacct tcggtttgat cttctcttgc tgctgctgac tgtagccgag cgtgcgtaga
      181 tccacctggt cgacggtctt ctgcttcttg gtgtccacgt cgtcatcttc atcgtcctca
      241 gccctcttgc cagatctcat ggtggcggct gccaaagttg agcgtttatt ctgagcttct
      301 gcaaaaagaa caagcaagct tagctccagc ttttgttccc tatagtgagt cgtattatta
      361 attaaggatc tgtaggaaaa agaagaaggc atgaacatgg ttagcagagg ctctagagcc
      421 gccggtcaca cgccagaagc cgaaccccgc cctgccccgt cccccccgaa
//
