LOCUS       sequence-72456-e         796 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Provides a kanamycin resistance cassette as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGF007
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        121..300
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(388..407)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcaactaga gaattaaggg
       61 agtcacgtta tgacccccgc cgatgacgcg ggacaagccg ttttacgttt ggaactgaca
      121 gaaccgcaac gttgaaggag ccactgagcc gcgggtttct ggagtttaat gagctaagca
      181 catacgtcag aaaccattat tgcgcgttca aaagtcgcct aaggtcacta tcagctagca
      241 aatatttctt gtcaaaaatg ctccactgac gttccataaa ttcccctcgg tatccaatta
      301 gagtctcata ttcactctca actcgatcga ggcatgattg aacaagatgg attgcacgca
      361 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
      421 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
      481 aagaccgacc tgtccggtgc cctgaatgaa ctccaagacg aggcagcgcg gctatcgtgg
      541 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
      601 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
      661 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
      721 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
      781 gccggtcttg tcgatc
//
