LOCUS       sequence-7261-ex       10713 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Wiznerowicz M, Trono D
  TITLE     Conditional suppression of cellular genes: lentivirus 
            vector-mediated drug-inducible RNA interference.
  JOURNAL   J Virol. 2003 Aug . 77(16):8957-61.
  PUBMED    12885912
REFERENCE   2  (bases 1 to 10713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2003 Aug . 77(16):8957-61."
COMMENT     Sequence Label: pLV-tTRKRAB
FEATURES             Location/Qualifiers
     source          1..10713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3297
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3288
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3245..3265
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3345..3462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             3512..4132
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3585..3604)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             4133..4153
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4256..4450
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     misc_feature    4543..5131
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4596..4616)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5014..5025)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5275..5308)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5328..5508
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5533..5551)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5534..5551)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5841..5860)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6074..6093)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6193..6215
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6253..6271)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6339..6443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6444..6512,6513..7304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6662..6681)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7475..8063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7964..7983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8217..8234
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8304..8324)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8309..8638
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8489..8624
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9772..9837
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9967..9987
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10412..10546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10449..10468)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10503..10522
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttccgatcac gagactagcc
     3481 tcgaggttta aactacggga tctacgcgtc catggctaga ttagataaaa gtaaagtgat
     3541 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     3601 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
     3661 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     3721 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
     3781 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
     3841 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
     3901 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
     3961 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
     4021 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
     4081 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt cgccaaaaaa
     4141 gaagagaaag gtcgacggcg gtggtgcttt gtctcctcag cactctgctg tcactcaagg
     4201 aagtatcatc aagaacaagg agggcatgga tgctaagtca ctaactgcct ggtcccggac
     4261 actggtgacc ttcaaggatg tatttgtgga cttcaccagg gaggagtgga agctgctgga
     4321 cactgctcag cagatcgtgt acagaaatgt gatgctggag aactataaga acctggtttc
     4381 cttgggttat cagcttacta agccagatgt gatcctccgg ttggagaagg gagaagagcc
     4441 ctggctggtg gagagagaaa ttcaccaaga gacccatcct gattcagaga ctgcatttga
     4501 aatcaaatca tcagtttaac ccgggtatac tagtcatatg ataatcaacc tctggattac
     4561 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4621 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4681 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4741 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4801 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4861 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4921 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     4981 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5041 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5101 agtcggatct ccctttgggc cgcctccccg catcggtacg tatggccagg tacctttaag
     5161 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     5221 ggaagggcta attcactccc aacgaagaca agatgggatc aattcaccat gggaataact
     5281 tcgtatagca tacattatac gaagttatgc tgctttttgc ttgtactggg tctctctggt
     5341 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5401 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5461 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt
     5521 aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta
     5581 ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt
     5641 actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg
     5701 aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag
     5761 cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     5821 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     5881 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     5941 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     6001 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat
     6061 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     6121 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     6181 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     6241 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     6301 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6361 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6421 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6481 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6541 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6601 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6661 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6721 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6781 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6841 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6901 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6961 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7021 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7081 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7141 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7201 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7261 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7321 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7381 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7441 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7501 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7561 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7621 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7681 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7741 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7801 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7861 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7921 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7981 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8041 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8101 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8161 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8221 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8281 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     8341 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     8401 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8461 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8521 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     8581 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     8641 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
     8701 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
     8761 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
     8821 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
     8881 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
     8941 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     9001 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     9061 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     9121 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
     9181 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
     9241 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     9301 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
     9361 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     9421 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     9481 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     9541 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     9601 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     9661 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
     9721 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     9781 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     9841 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     9901 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     9961 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
    10021 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
    10081 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
    10141 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
    10201 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
    10261 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
    10321 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    10381 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    10441 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    10501 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
    10561 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    10621 agtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
    10681 gtatttgtta aatatgtact acaaacttag tag
//
