LOCUS       sequence-72782-e         815 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Bonnet J, Subsoontorn P, Endy D
  TITLE     Rewritable digital data storage in live cells via engineered control
            of recombination directionality.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 
            21.
  PUBMED    22615351
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-06-5"; volume: "109(23)"; pages: 
            "8884-9. Epub 2012 May 21"
COMMENT     Sequence Label: pSB4A5 JQ929582
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(4..50)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(74..787)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggct
       61 ctagatatca tcatttgtac agttcatcca taccatgcgt gatgcccgct gcggttacga
      121 actccagcag aaccatatga tcgcgtttct cgttcggatc tttagacaga acgctttgcg
      181 tgctcagata gtgattgtct ggcagcagaa caggaccatc accgattgga gtgttttgct
      241 ggtagtgatc agccagctgc acgctgccat cctccacgtt gtggcgaatt ttaaaattcg
      301 ctttaatgcc atttttttgt ttatcggcgg tgatgtaaac attgtggctg ttaaaattgt
      361 attccagctt atggcccagg atattgccgt cttctttaaa gtcaatgcct ttcagctcaa
      421 tgcggtttac cagggtatcg ccttcaaatt tcacttccgc acgcgttttg tacgtgccgt
      481 catccttaaa ggaaatcgtg cgttcctgca catagccttc cggcatggcg gacttgaaga
      541 agtcatgctg cttcatatgg tccggataac gagcaaagca ctgaacacca taagtcagcg
      601 tcgttaccag agtcggccaa ggtaccggca gtttaccagt agtacagatg aacttcagcg
      661 tcagtttacc attagttgcg tcaccttcac cctcgccacg cacggaaaac ttatgaccgt
      721 tgacatcacc atccagttcc accagaatag ggacgacacc agtgaacagc tcttcgcctt
      781 tacgcatatg tatatctcct tcttaaaaga tcttt
//
