LOCUS       sequence-72886-e         990 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Peroxide-insensitive version of HyPer to be used as a pH-control. 
            Can be applied as a pH sensor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Belousov VV, Fradkov AF, Lukyanov KA, Staroverov DB, Shakhbazov KS, 
            Terskikh AV, Lukyanov S
  TITLE     Genetically encoded fluorescent indicator for intracellular hydrogen
            peroxide.
  JOURNAL   Nat Methods. 2006 Apr;3(4):281-6.
  PUBMED    16554833
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2006-04"; volume: "3(4)"; pages: "281-6"
COMMENT     Sequence Label: pC1-HyPer-C199S
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     645..666
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(775..796)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gccaccatgg agatggcaag ccagcagggc
       61 gagacgatgt ccggaccgct gcacattggt ttgattccca cagttggacc gtacctgcta
      121 ccgcatatta tccctatgct gcaccagacc tttccaaagc tggaaatgta tctgcatgaa
      181 gcacagaccc accagttact ggcgcaactg gacagcggca aactcgattg cgtgatcctc
      241 gcgctggtga aagagagcga agcattcatt gaagtgccgt tgtttgatga gccaatgttg
      301 ctggctatct atgaagatca cccgtgggcg aaccgcgaat gcgtaccgat ggccgatctg
      361 gcaggggaaa aactgctgat gctggaagat ggtcactctt tgcgcgatca ggcaatgtcc
      421 gccggctaca acagcgacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
      481 gccaacttca agatccgcca caacgtcgag gacggcagcg tgcagctcgc cgaccactac
      541 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      601 ttccagtccg tcctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      661 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaacgt ggatggcggt
      721 agcggtggca ccggcagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      781 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      841 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
      901 tggcccaccc tcgtgaccac cctcggctac ggcctgaagt gcttcgcccg ctaccccgac
      961 cacatgaagc agcacgactt cttcaagtcc
//
