LOCUS       sequence-72958-e         859 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Trasgenically expresses tandem tdtomato fluorescent protein in T. 
            cruzi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Canavaci AM, Bustamante JM, Padilla AM, Perez Brandan CM, Simpson 
            LJ, Xu D, Boehlke CL, Tarleton RL
  TITLE     In vitro and in vivo high-throughput assays for the testing of 
            anti-Trypanosoma cruzi compounds.
  JOURNAL   PLoS Negl Trop Dis. 2010 Jul 13;4(7):e740. doi: 
            10.1371/journal.pntd.0000740.
  PUBMED    20644616
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2010-07-13"; volume: "4(7):e740. doi"; pages: " 
            10.1371/journal.pntd.0000740"
COMMENT     Sequence Label: pTrex-Neo-tdTomato
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(91..114)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(289..308)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     558..576
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tgccgctgcc ggtgctgccg gtgccatgcc ccaggaacag gtggtggcgg ccctcggagc
       61 gctcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc agcttggtgt
      121 ccacgtagta gtagccgggc agttgcacgg gcttcttggc catgtagatg gtcttgaact
      181 ccaccaggta gtggccgccg tccttcagct tcagggcctg gtggatctcg cccttcagca
      241 cgccgtcgcg ggggtacagg cgctcggtgg aggcctccca gcccatggtc ttcttctgca
      301 ttacggggcc gtcggggggg aagttggtgc cgcgcatctt caccttgtag atcagcgtgc
      361 cgtcctgcag ggaggagtcc tgggtcacgg tcaccagacc gccgtcctcg aagttcatca
      421 cgcgctccca cttgaagccc tcggggaagg acagcttctt gtaatcgggg atgtcggcgg
      481 ggtgcttcac gtacgccttg gagccgtaca tgaactgggg ggacaggatg tcccaggcga
      541 agggcagggg gccgcccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
      601 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt catggagccc tccatgcgca
      661 ccttgaagcg catgaactct ttgatgacct cctcgccctt gctcacccta ggcgccatgc
      721 tagcagatct aaaagggaat tctagacaag acaacttata gagcaaaaaa aaaaaagaaa
      781 agacatcata aaagaagttg gaaaagaaaa aaggtaaaaa caatttaaca caacaataat
      841 aagtggataa aacaacaaa
//
