LOCUS       sequence-73051-e         837 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses V591A and L686E mutated Ataxin1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Kim E, Lu HC, Zoghbi HY, Song JJ
  TITLE     Structural basis of protein complex formation and reconfiguration by
            polyglutamine disease protein Ataxin-1 and Capicua.
  JOURNAL   Genes Dev. 2013 Mar 15;27(6):590-5. doi: 10.1101/gad.212068.112.
  PUBMED    23512657
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.212068"; journalName: "Genes Dev"; date: "2013-03-15- 
            15"; volume: "27"; issue: "6"; pages: "590-5"
COMMENT     Sequence Label: Flag-ATXN1_V591A_L686E
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             198..221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattccgtga
      121 ctttccgttt atcagactaa aatcagagcc atccagacag tgaaacagtc accgtggagg
      181 ggggacggcg aaaaatggac tacaaagacg acgacgacaa aaaatccaac caagagcgga
      241 gcaacgaatg cctgcctccc aagaagcgcg agatccccgc caccagccgg tcctccgagg
      301 agaaggcccc taccctgccc agcgacaacc accgggtgga gggcacagca tggctcccgg
      361 gcaaccctgg tggccggggc cacgggggcg ggaggcatgg gccggcaggg acctcggtgg
      421 agcttggttt acaacaggga ataggtttac acaaagcatt gtccacaggg ctggactact
      481 ccccgcccag cgctcccagg tctgtccccg tggccaccac gctgcctgcc gcgtacgcca
      541 ccccgcagcc agggaccccg gtgtcccccg tacagtacgc tcacctgccg cacaccttcc
      601 agttcattgg gtcctcccaa tacagtggaa cctatgccag cttcatccca tcacagctga
      661 tccccccaac cgccaacccc gtcaccagtg cagtggcctc ggccgcaggg gccaccactc
      721 catcccagcg ctcccagctg gaggcctatt ccactctgct ggccaacatg ggcagtctga
      781 gccagacgcc gggacacaag gctgagcagc agcacctcag cagggctccg gggctta
//
