LOCUS       sequence-73125-e        1013 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Human codon optimized Cas9 nuclease from Streptococcus pyogenes 
            (Cbh-3X-FLAG-NLS-SpCas9-NLS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
  TITLE     Genome engineering using the CRISPR-Cas9 system.
  JOURNAL   Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. 
            Epub 2013 Oct 24.
  PUBMED    24157548
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.143"; journalName: "Nat Protoc"; date: 
            "2013-11"; volume: "8"; issue: "11"; pages: "2281-308"
COMMENT     Sequence Label: pSpCas9 (PX165)
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..61)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctaggaattc ttactttttc ttttttgcct ggccggcctt tttcgtggcc gccggccttt
       61 tgtcgcctcc cagctgagac aggtcgatcc gtgtctcgta caggccggtg atgctctggt
      121 ggatcagggt ggcgtccagc acctctttgg tgctggtgta cctcttccgg tcgatggtgg
      181 tgtcaaagta cttgaaggcg gcaggggctc ccagattggt cagggtaaac aggtggatga
      241 tattctcggc ctgctctctg atgggcttat cccggtgctt gttgtaggcg gacagcactt
      301 tgtccagatt agcgtcggcc aggatcactc tcttggagaa ctcgctgatc tgctcgatga
      361 tctcgtccag gtagtgcttg tgctgttcca caaacagctg tttctgctca ttatcctcgg
      421 gggagccctt cagcttctca tagtggctgg ccaggtacag gaagttcaca tatttggagg
      481 gcagggccag ttcgtttccc ttctgcagtt cgccggcaga ggccagcatt ctcttccggc
      541 cgttttccag ctcgaacagg gagtacttag gcagcttgat gatcaggtcc tttttcactt
      601 ctttgtagcc cttggcttcc agaaagtcga tgggattctt ctcgaagctg cttctttcca
      661 tgatggtgat ccccagcagc tctttcacac tcttcagttt cttggacttg cccttttcca
      721 ctttggccac caccagcaca gaataggcca cggtggggct gtcgaagccg ccgtacttct
      781 tagggtccca gtccttcttt ctggcgatca gcttatcgct gttcctcttg ggcaggatag
      841 actctttgct gaagccgcct gtctgcacct cggtcttttt cacgatattc acttggggca
      901 tgctcagcac tttccgcacg gtggcaaaat cccggccctt atcccacacg atctccccgg
      961 tttcgccgtt tgtctcgatc agaggccgct tccggatctc gccgttggcc agg
//
