LOCUS       sequence-73129-e         972 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  FX sequencing vector for subcloning, tetracycline marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pINIT_tet
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(172..258)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
       61 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgact agaagagcgg
      121 ccaaccaggc cgtgcagcgc ttgcggccgg ccgctaggtc tagggcggcg gatttgtcct
      181 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg
      241 agcctttcgt tttatttgat gcctctagac gacccaccgc cgagaccgcg gccgcaatgc
      301 caataggata tcggcatttt cttttgcgtt tttatttgtt aactgttaat tgtccttgtt
      361 caaggatgct gtctttgaca acagatgttt tcttgccttt gatgttcagc aggaagctag
      421 gcgcaaacgt tgattgtttg tctgcgtaga atcctctgtt tgtcatatag cttgtaatca
      481 cgacattgtt tcctttcgct tgaggtacag cgaagtgtga gtaagtaaag gttacatcgt
      541 taggatcaag atccattttt aacacaaggc cagttttgtt cagcggcttg tatgggccag
      601 ttaaagaatt agaaacataa ccaagcatgt aaatatcgtt agacgtaatg ccgtcaatcg
      661 tcatttttga tccgcgggag tcagtgaaca ggtaccattt gccgttcatt ttaaagacgt
      721 tcgcgcgttc aatttcatct gttactgtgt tagatgcaat cagcggtttc atcacttttt
      781 tcagtgtgta atcatcgttt agctcaatca taccgagagc gccgtttgct aactcagccg
      841 tgcgtttttt atcgctttgc agaagttttt gactttcttg acggaagaat gatgtgcttt
      901 tgccatagta tgctttgtta aataaagatt cttcgccttg gtagccatct tcagttccag
      961 tgtttgcttc aa
//
