LOCUS       sequence-73168-e         850 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, 
            Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Punna T, Isserlin R, 
            Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J
  TITLE     A lentiviral-based functional proteomics approach identifies 
            chromatin remodelling complexes important for the induction of 
            pluripotency.
  JOURNAL   Mol Cell Proteomics. 2010 May;9(5):811-23.
  PUBMED    20305087
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2010-05"; volume: "9(5)"; pages: "811-23"
COMMENT     Sequence Label: pLD-puro-PGKnVA
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..90
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             100..120
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             133..153
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             166..183
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             196..219
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             256..279
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    361..485
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(510..515,516..533,534..540)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(660..679)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccgggccttt cggctagcac catggactac aaagaccatg acggtgatta taaagatcat
       61 gacatcgatt acaaggatga cgatgacaag cctgcaggag aaaatctgta cttccagggg
      121 agaggaggca gcgagaacct gtactttcag gggcctgcag gaagacatca ccatcaccat
      181 cacagcggcg gaagatggag ccaccctcag ttcgagaagg gaggaggaag cggcggaggc
      241 agcggaggag gaagctggag ccacccgcag ttcgagaaag cgatcgccag agagctgttc
      301 aacctgctgg gcgagaacca gccccccgtg gtgatcaagg cgatcgccac cggtaagctt
      361 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
      421 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      481 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      541 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      601 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      661 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      721 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      781 ttgcccgcct gatgaatgct catccggaat tccgtatggc atgaaagacg gtgagctggt
      841 gatatgggat
//
