LOCUS       sequence-73171-e         918 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  FX cloning Saccharomyces cerevisiae expression vector with GAL1 
            promoter and N-terminal 10x His tag, GFP and 3C protease cleavage 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pYEXNHG3
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(90..96,97..114,115..120))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(145..269)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(288..308)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(312..332)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             386..427
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             437..454
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      488..735
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(504..522)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(812..829)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
       61 tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa
      121 gcctggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat
      181 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
      241 tctcgttcag cttttttgta caaacttgtg atatcgaatt cgctagctac ctttctcttc
      301 ttttttggat ctacctttct cttctttttt ggggatccac tagttcagct cttctgcata
      361 ataactatct agagggccct tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
      421 ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct gatcctagag
      481 ggccgcatca tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct
      541 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata
      601 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga
      661 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg
      721 aaggctttaa tttgcaagct gcggccctgc attaatgaat cggccaacgc gcggggagag
      781 gcggtttgcg tattgggcgc tattccgctt cctcgctcac tgactcgctg cgctcggtcg
      841 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      901 caggggataa cgcaggaa
//
