LOCUS       sequence-73284-e        1009 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Used as a PCR template for tagging yeast proteins with the SNAP tag 
            by homologous recombination and selection for ClonNAT resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, 
            Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ
  TITLE     Ordered and dynamic assembly of single spliceosomes.
  JOURNAL   Science. 2011 Mar 11;331(6022):1289-95.
  PUBMED    21393538
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-03-11"; volume: "331(6022)"; pages: "1289-95"
COMMENT     Sequence Label: pAAH6
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(32..229)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(235..807)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
ORIGIN
        1 ctggatggcg gcgttagtat cgaatcgaca gcagtatagc gaccagcatt cacatacgat
       61 tgacgcatga tattactttc tgcgcactta acttcgcatc tgggcagatg atgtcgaggc
      121 gaaaaaaaat ataaatcacg ctaacatttg attaaaatag aacaactaca atataaaaaa
      181 actatacaaa tgacaagttc ttgaaaacaa gaatcttttt attgtcagta ctgattaggg
      241 gcagggcatg ctcatgtaga gcgcctgctc gccgtccgag gcggtgccgt cgtacagggc
      301 ggtgtccagg ccgcagaggg tgaaccccat ccgccggtac gcgtggatcg ccggtgcgtt
      361 gacgttggtg acctccagcc agaggtgccc ggcgccccgc tcgcgggcga actccgtcgc
      421 gagccccatc aacgcgcgcc cgaccccgtg cccccggtgc tccggggcga cctcgatgtc
      481 ctcgacggtc agccggcggt tccagccgga gtacgagacg accacgaagc ccgccaggtc
      541 gccgtcgtcc ccgtacgcga cgaacgtccg ggagtccggg tcgccgtcct ccccgtcgtc
      601 cgattcgtcg tccgattcgt cgtcggggaa caccttggtc aggggcgggt ccaccggcac
      661 ctcccgcagg gtgaagccgt ccccggtggc ggtgacgcgg aagacggtgt cggtggtgaa
      721 ggacccatcc agtgcctcga tggcctcggc gtcccccggg acactggtgc ggtaccggta
      781 agccgtgtcg tcaagagtgg tacccatggt tgtttatgtt cggatgtgat gtgagaactg
      841 tatcctagca agattttaaa aggaagtata tgaaagaaga acctcagtgg caaatcctaa
      901 ccttttatat ttctctacag gggcgcggcg tggggacaat tcaacgcgtc tgtgagggga
      961 gcgtttccct gctcgcaggt ctgcagcgag gagccgtaat ttttgcttc
//
