LOCUS       sequence-73361-e         752 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  FX cloning Xenopus oocyte expression vector with SP6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pTLNX
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(91..97,98..115,116..121))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(146..270)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(289..309)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(313..333)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     547..563
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     548..564
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     3'UTR           614..739
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
ORIGIN
        1 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
       61 atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa
      121 agcctggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta
      181 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
      241 ttctcgttca gcttttttgt acaaacttgt gatatcgaat tcgctagcta cctttctctt
      301 cttttttgga tctacctttc tcttcttttt tggggatcca ctagttcagc tcttctgcat
      361 aatccggtct gaaggaggtg gctcgtctcg ggggctgggg atgggatggt gggtgttgga
      421 tgcatctccc gggatttatc ttgagaatat aaagacccag aggttgagat agagagaaag
      481 aaagatcggg tcgaccacga attcttgctc caggtccaga caggacgctt tagagcacaa
      541 tggatctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag cccgggggat
      601 ctggttacca ctaaaccagc ctcaagaaca cccgaatgga gtctctaagc tacataatac
      661 caacttacac tttacaaaat gttgtccccc aaaatgtagc cattcgtatc tgctcctaat
      721 aaaaagaaag tttcttcaca ttctaaaaaa aa
//
