LOCUS       sequence-73729-e         771 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses AmCyan while the mCherry gene has been mutated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  TITLE     AmCyan-P2A-Xerry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AmCyan-P2A-Xerry
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    118..174
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(466..485)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     734..752
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
       61 aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag atccggtgga
      121 tcccgggccc gcggtaccgt cgactgcaga attcgaagct tgagctcgag atctgagact
      181 agtttccgga cttgtacagc tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt
      241 cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga
      301 cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc ttgacctcag
      361 cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc ttcagggcgc
      421 cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc ttctgcatta
      481 cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg aactcgccgt
      541 cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc
      601 gctcccactt gaagccctcg gggaaggaca gcttcaagta gtcggggatg tcggcggggt
      661 gcttcacgta ggccttggag cctacatgaa ctgaggggac aggatgtccc aggcgaaggg
      721 cagggggcca cccttggtca ccttcagctt ggcggtctgg gtgccctcgt a
//
