LOCUS       sequence-73805-e         832 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Wu S, Ying G, Wu Q, Capecchi MR
  TITLE     A protocol for constructing gene targeting vectors: generating 
            knockout mice for the cadherin family and beyond.
  JOURNAL   Nat Protoc. 2008  . 3(6):1056-76.
  PUBMED    18546598
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2008  . 3(6):1056-76."
COMMENT     Sequence Label: pWS-TK6
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    228..352
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(377..382,383..400,401..407)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(527..546)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggcgcgcctg caggcgcgcc actagttaat tatttaaatc gatgcgacgc tagcggccgc
       61 gtttgctttt gtttcgtgtt ggtcaccacg gccgagtttc ctcgggaccc cgggcagctg
      121 ctttacatcc caaagaccta tctgctcggc cggcctcgga acgcgagcct gcccggaaga
      181 tccccgggta ccgagctcga attcatcgtc accatcacct cgaatcaaca agtttgtaca
      241 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
      301 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
      361 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
      421 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
      481 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
      541 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
      601 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
      661 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
      721 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
      781 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tg
//
