LOCUS       sequence-73841-e         865 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pMT mir-1-1 reporter cel-lsy-6
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(340..364)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             378..419
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             778..795
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(818..835)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 aaaaagcagg ctcatatggc aattgagcaa agcatctgca ggtgcctgat attttacggc
       61 ttccgcccat taccgtagct gaaagagtat aaatagaaga ttcatcgttc aaatgtttaa
      121 tgttcgactt ttcaaataat gtattcctac taatttctga aaaataagcc atcaaatgcg
      181 tctagtatca aaatcatgta aaaactgtaa aacagatttt gtatgagacg catttcgatg
      241 acgggcacaa cagagacacc gcccataaga cggtatcttt tgatggtaag aggtgtgaat
      301 ctataatacg actgtgtgat ccatgtgtcg gttaagctta cccagctttc ttgtacaaag
      361 tggttcgatg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
      421 gttctagagc agtggggtca gcttctaccg gggcggcgtc ccggggtctt ggaactgcat
      481 gcagactgcc tgcttgggaa acatacttct ttatatgccc atatggacct gctaagctat
      541 ggaatgtaaa gaagtatgta tctcaggccg ggacctctct cgccgcactg aggggcactc
      601 cacaccacgg gggccgccgc ggccgacagc cagccaggcg gctgtggtgg gggtgaggct
      661 ggggcggcag gctgcctggg cgttggtgga cggtcgtgtc cccgacagca ttgttctgtc
      721 actttgcctg tgtcagcagg tggaagtgct caccagacat ctagaacgcg taccggtcat
      781 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctaagatc
      841 cagacatgat aagatacatt gatga
//
