LOCUS       sequence-73944-e        7918 bp    DNA     circular SYN 15-APR-2025
DEFINITION  expresses GAL4BD-Rab1AQ70Ldelta CC in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Cortes C, Rzomp KA, Tvinnereim A, Scidmore MA, Wizel B
  TITLE     Chlamydia pneumoniae inclusion membrane protein Cpn0585 interacts 
            with multiple Rab GTPases.
  JOURNAL   Infect Immun. 2007 Dec;75(12):5586-96. Epub 2007 Oct 1.
  PUBMED    17908815
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2007-12"; volume: "75(12)"; pages: "5586-96. Epub 
            2007 Oct 1"
COMMENT     Sequence Label: GBK-Rab1AQ70LdeltaCC
FEATURES             Location/Qualifiers
     source          1..7918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      1952..1999
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2026..2213
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2081..2105)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2237..2253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2237..2253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2250..2272)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2261..2277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2285..2291,2292..2309,2310..2315))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(2468..2485)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2639..3227)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2719..2738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3556..3603
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3628..3647
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3836..4630)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3947..3966)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4557..4576
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4631..4735)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4762..6104
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(5725..5772)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     6145..6163
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6201..6223)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6323..6342
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6363..6644
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6645..7319
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7422..7877)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7559..7580)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7771..7790
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga
      421 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg
      481 atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct
      541 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcccgggg atccttatgt
     1321 ccagcatgaa tcccgaatat gattatttat tcaagttact tctgattggc gactcagggg
     1381 ttggaaagtc ttgccttctt cttaggtttg cagatgatac atatacagaa agctacatca
     1441 gcacaattgg tgtggatttc aaaataagaa ctatagagtt agacgggaaa acaatcaagc
     1501 ttcaaatatg ggacacagca ggcctggaaa gatttcgaac aatcacctcc agttattaca
     1561 gaggagccca tggcatcata gttgtgtatg atgtgacaga tcaggagtcc ttcaataatg
     1621 ttaaacagtg gctgcaggaa atagatcgtt atgccagtga aaatgtcaac aaattgttgg
     1681 tagggaacaa atgtgatctg accacaaaga aagtagtaga ctacacaaca gcgaaggaat
     1741 ttgctgattc ccttggaatt ccgtttttgg aaaccagtgc taagaatgca acgaatgtag
     1801 aacagtcttt catgacgatg gcagctgaga ttaaaaagcg aatgggtccc ggagcaacag
     1861 ctggtggtgc tgagaagtcc aatgttaaaa ttcagagcac tccagtcaag cagtcaggtg
     1921 gaggttaact cgacctgcag cggccgcata actagcataa ccccttgggg cctctaaacg
     1981 ggtcttgagg ggttttttgc gcgcttgcag ccaagctaat tccgggcgaa tttcttatga
     2041 tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt
     2101 gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg
     2161 ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgcaa
     2221 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2281 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt
     2341 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2401 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2461 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2521 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2581 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2641 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2701 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2761 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2821 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2881 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2941 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3001 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3061 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3121 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3181 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3241 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3301 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3361 caatctaaag tatatatgag taacctgagg ctatggcagg gcctgccgcc ccgacgttgg
     3421 ctgcgagccc tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc
     3481 gggcgtattg gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc
     3541 gaaccccgcg tttatgaaca aacgacccaa caccgtgcgt tttattctgt ctttttattg
     3601 ccgtcatagc gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc
     3661 tagggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt
     3721 cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc
     3781 gtgatggcag gttgggcgtc gcttggtcgg ttcatttcga accccagagt cccgctcaga
     3841 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
     3901 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
     3961 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
     4021 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgtcatgg gtcacgacga
     4081 gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc
     4141 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     4201 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     4261 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     4321 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     4381 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     4441 cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     4501 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     4561 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     4621 gttcaatcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4681 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4741 ttccccgaaa agtgccacct gaacgaagca tctgtgcttc attttgtaga acaaaaatgc
     4801 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     4861 tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa
     4921 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
     4981 agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct
     5041 acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc
     5101 tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag
     5161 gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact
     5221 tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc
     5281 ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt
     5341 tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac
     5401 tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt
     5461 actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg
     5521 agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca
     5581 cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata
     5641 ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc
     5701 gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagagaata ggaacttcgg
     5761 aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg
     5821 cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata
     5881 catgagaaga acggcatagt gcgtgtttat gcttaaatgc gtacttatat gcgtctattt
     5941 atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat
     6001 cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga
     6061 ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcatatta agaaaccatt
     6121 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     6181 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     6241 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     6301 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccataga
     6361 tcaacgacat tactatatat ataatatagg aagcatttaa tagaacagca tcgtaatata
     6421 tgtgtacttt gcagttatga cgccagatgg cagtagtgga agatattctt tattgaaaaa
     6481 tagcttgtca ccttacgtac aatcttgatc cggagctttt ctttttttgc cgattaagaa
     6541 ttaattcggt cgaaaaaaga aaaggagagg gccaagaggg agggcattgg tgactattga
     6601 gcacgtgagt atacgtgatt aagcacacaa aggcagcttg gagtatgtct gttattaatt
     6661 tcacaggtag ttctggtcca ttggtgaaag tttgcggctt gcagagcaca gaggccgcag
     6721 aatgtgctct agattccgat gctgacttgc tgggtattat atgtgtgccc aatagaaaga
     6781 gaacaattga cccggttatt gcaaggaaaa tttcaagtct tgtaaaagca tataaaaata
     6841 gttcaggcac tccgaaatac ttggttggcg tgtttcgtaa tcaacctaag gaggatgttt
     6901 tggctctggt caatgattac ggcattgata tcgtccaact gcatggagat gagtcgtggc
     6961 aagaatacca agagttcctc ggtttgccag ttattaaaag actcgtattt ccaaaagact
     7021 gcaacatact actcagtgca gcttcacaga aacctcattc gtttattccc ttgtttgatt
     7081 cagaagcagg tgggacaggt gaacttttgg attggaactc gatttctgac tgggttggaa
     7141 ggcaagagag ccccgaaagc ttacatttta tgttagctgg tggactgacg ccagaaaatg
     7201 ttggtgatgc gcttagatta aatggcgtta ttggtgttga tgtaagcgga ggtgtggaga
     7261 caaatggtgt aaaagactct aacaaaatag caaatttcgt caaaaatgct aagaaatagg
     7321 ttattactga gtagtattta tttaagtatt gtttgtgcac ttgccgatct atgcggtgtg
     7381 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa acgttaatat
     7441 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     7501 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     7561 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     7621 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     7681 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     7741 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     7801 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     7861 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgat
//
