LOCUS       sequence-74287-e       11391 bp    DNA     circular SYN 14-APR-2025
DEFINITION  expresses VSVG fused to UVR8 for light-triggered trafficking.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11391)
  AUTHORS   Chen D, Gibson ES, Kennedy MJ
  TITLE     A light-triggered protein secretion system.
  JOURNAL   J Cell Biol. 2013 May 13;201(4):631-40. doi: 10.1083/jcb.201210119.
  PUBMED    23671313
REFERENCE   2  (bases 1 to 11391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-05-13- 13"; volume: "201"; 
            issue: "4"; pages: "631-40"
COMMENT     Sequence Label: VSVG-YFP-3xUVR8
FEATURES             Location/Qualifiers
     source          1..11391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        323..526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     477..497
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     523..547
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           616..655
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             join(678..725,726..2210)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein, 
                     thermosensitive tsO45 mutant"
                     /label=VSV-G tsO45
                     /note="thermosensitive variant of VSV-G Orsay"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAAIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNDICPTVHNSTTWHSDYKVKGLCDSNLISTDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGDKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPVFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSLGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDETLFFGDTGLSKNPIEFVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIYLCIKLKHTKKRQIYTDIEMNRLGK"
     CDS             2223..2252
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2265..2274
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2271..2273,2274..2276,2277..2984)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(2316..2337)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2577..2596)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2924..2945
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      3018..3027
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7035..7061
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          7093..7665
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(7797..7816)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7862..7917
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7916..7935)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      8270..8405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8511..8528)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8682..9270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8762..8781)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10064..10083
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10314..10418)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10486..10504
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10542..10564)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10664..10683
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(10750..11205)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10887..10908)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11099..11118
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11335..11357
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11349..11366
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11350..11366
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11373..11391
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcgttcga gctcgccccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
       61 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      121 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      181 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      241 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      301 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      361 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      421 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      481 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
      541 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
      601 atccagcctc cgggggatct tggtggcgtg aaactcccgc acctcttcgg ccagcgaatt
      661 caagcttcga attcgccatg aagtgccttt tgtacttagc ttttttattc atcggggtga
      721 attgcaagtt caccatagtt tttccacaca accaaaaagg aaactggaaa aatgttcctt
      781 ccaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac ttaataggca
      841 cagccttaca agtcaaaatg cccaagagtc acaaggctat tcaagcagac ggttggatgt
      901 gtcatgcttc caaatgggtc actacttgtg atttccgctg gtacggaccg aagtatataa
      961 cacattccat ccgatccttc actccatctg tagaacaatg caaggaaagc attgaacaaa
     1021 cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga tatgcaactg
     1081 tgacggatgc tgaagcagcg attgtccagg tgactcctca ccatgtgctt gttgatgaat
     1141 acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat gacatatgcc
     1201 ccactgtcca taactccaca acctggcatt ccgactataa ggtcaaaggg ctatgtgatt
     1261 ctaacctcat ttccacggac atcaccttct tctcagagga cggagagcta tcatccctag
     1321 gaaaggaggg cacagggttc agaagtaact actttgctta tgaaactgga gacaaggcct
     1381 gcaaaatgca gtactgcaag cattggggag tcagactccc atcaggtgtc tggttcgaga
     1441 tggctgataa ggatctcttt gctgcagcca gattccctga atgcccagaa gggtcaagta
     1501 tctctgctcc atctcagacc tcagtggatg taagtctcat tcaggacgtt gagaggatct
     1561 tggattattc cctctgccaa gaaacctgga gcaaaatcag agcgggtctt cccatctctc
     1621 cagtggatct cagctatctt gctcctaaaa acccaggaac cggtcctgtc tttaccataa
     1681 tcaatggtac cctaaaatac tttgagacca gatacatcag agtcgatatt gctgctccaa
     1741 tcctctcaag aatggtcgga atgatcagtg gaactaccac agaaagggaa ctgtgggatg
     1801 actgggctcc atatgaagac gtggaaattg gacccaatgg agttctgagg accagtttag
     1861 gatataagtt tcctttatat atgattggac atggtatgtt ggactccgat cttcatctta
     1921 gctcaaaggc tcaggtgttt gaacatcctc acattcaaga cgctgcttcg cagcttcctg
     1981 atgatgagac tttatttttt ggtgatactg ggctatccaa aaatccaatc gagtttgtag
     2041 aaggttggtt cagtagttgg aagagctcta ttgcctcttt tttctttatc atagggttaa
     2101 tcattggact attcttggtt ctccgagttg gtatttatct ttgcattaaa ttaaagcaca
     2161 ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag gatctccccg
     2221 ccgaacagaa actcatctcc gaagaagacc tggatccacc ggtcgccacc atggtgagca
     2281 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     2341 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     2401 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2461 ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     2521 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     2581 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2641 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2701 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     2761 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     2821 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     2881 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2941 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacagtggt ggtggagggg
     3001 ggggactcag atctcgagcc accatggcgg aggatatggc tgccgacgaa gttacggctc
     3061 ctcctcgtaa ggttcttatc atctccgctg gtgctagcca ctccgtcgct cttctctctg
     3121 gtgacattgt ttgttcttgg ggtcgaggag aggatggaca gttaggtcat ggcgatgcag
     3181 aggatcgacc ttctccgact cagcttagcg ctttagatgg ccaccaaatt gtttccgtta
     3241 cctgtggtgc tgatcacact gttgcttatt cacaatcagg catggaagtc tacagttggg
     3301 gatggggtga ttttgggaga ttaggccatg gtaactcaag cgacttgttt actccgctac
     3361 caatcaaagc attgcacggt attcggatca agcagattgc ttgtggggat agtcattgtt
     3421 tggctgtcac tatggaagga gaggtccaga gttggggccg caaccagaat ggtcaacttg
     3481 gtctggggga caccgaagat tctctagtgc ctcagaagat tcaagccttt gagggaatac
     3541 gaatcaaaat ggttgctgct ggtgcagaac acactgctgc agttacagaa gatggtgacc
     3601 tctatggatg gggctgggga agatacggaa atttgggatt aggtgaccgg actgaccgct
     3661 tagttcctga aagagttacc tctactggtg gtgagaaaat gtcaatggtt gcttgtggat
     3721 ggcggcacac aatatcagtt tcctactctg gagcattgta tacttatgga tggagcaaat
     3781 atggacagct aggacatgga gacttggagg atcaccttat tcctcacaaa ctggaagcac
     3841 tgagcaacag ttttatctcc cagatttcgg gaggttggag acatacaatg gcattgactt
     3901 cagatggaaa actatatgga tggggttgga ataagtttgg acaagtagga gtcggcaata
     3961 atttagatca gtgttctcct gtgcaagtgc gatttcccga tgatcagaaa gtagttcaag
     4021 tctcatgtgg atggagacat accttggctg tcactgaaag aaataacgtg tttgcttggg
     4081 gtagaggtac aaatggacag ctcggcattg gagagtcggt tgacaggaac tttcccaaga
     4141 ttatagaggc actcagcgtc gatggagcaa gtggacaaca tatagaatct tctaatatcg
     4201 atccatcttc agggaaaagc tgggtgtcgc ctgcagagag atatgcagtt gttcctgatg
     4261 aaacgggcct aacggatggt tcaagcaaag gtaatggagg tgatatcagt gttccacaaa
     4321 ctgatgtcaa gcgtgtacga attaagcttg ggggcggagg cgcggaggat atggctgccg
     4381 acgaagttac ggctcctcct cgtaaggttc ttatcatctc cgctggtgct agccactccg
     4441 tcgctcttct ctctggtgac attgtttgtt cttggggtcg aggagaggat ggacagttag
     4501 gtcatggcga tgcagaggat cgaccttctc cgactcagct tagcgcttta gatggccacc
     4561 aaattgtttc cgttacctgt ggtgctgatc acactgttgc ttattcacaa tcaggcatgg
     4621 aagtctacag ttggggatgg ggtgattttg ggagattagg ccatggtaac tcaagcgact
     4681 tgtttactcc gctaccaatc aaagcattgc acggtattcg gatcaagcag attgcttgtg
     4741 gggatagtca ttgtttggct gtcactatgg aaggagaggt ccagagttgg ggccgcaacc
     4801 agaatggtca acttggtctg ggggacaccg aagattctct agtgcctcag aagattcaag
     4861 cctttgaggg aatacgaatc aaaatggttg ctgctggtgc agaacacact gctgcagtta
     4921 cagaagatgg tgacctctat ggatggggct ggggaagata cggaaatttg ggattaggtg
     4981 accggactga ccgcttagtt cctgaaagag ttacctctac tggtggtgag aaaatgtcaa
     5041 tggttgcttg tggatggcgg cacacaatat cagtttccta ctctggagca ttgtatactt
     5101 atggatggag caaatatgga cagctaggac atggagactt ggaggatcac cttattcctc
     5161 acaaactgga agcactgagc aacagtttta tctcccagat ttcgggaggt tggagacata
     5221 caatggcatt gacttcagat ggaaaactat atggatgggg ttggaataag tttggacaag
     5281 taggagtcgg caataattta gatcagtgtt ctcctgtgca agtgcgattt cccgatgatc
     5341 agaaagtagt tcaagtctca tgtggatgga gacatacctt ggctgtcact gaaagaaata
     5401 acgtgtttgc ttggggtaga ggtacaaatg gacagctcgg cattggagag tcggttgaca
     5461 ggaactttcc caagattata gaggcactca gcgtcgatgg agcaagtgga caacatatag
     5521 aatcttctaa tatcgatcca tcttcaggga aaagctgggt gtcgcctgca gagagatatg
     5581 cagttgttcc tgatgaaacg ggcctaacgg atggttcaag caaaggtaat ggaggtgata
     5641 tcagtgttcc acaaactgat gtcaagcgtg tacgaattgg gtcgacgggt ggtggtgcgg
     5701 aggatatggc tgccgacgaa gttacggctc ctcctcgtaa ggttcttatc atctccgctg
     5761 gtgctagcca ctccgtcgct cttctctctg gtgacattgt ttgttcttgg ggtcgaggag
     5821 aggatggaca gttaggtcat ggcgatgcag aggatcgacc ttctccgact cagcttagcg
     5881 ctttagatgg ccaccaaatt gtttccgtta cctgtggtgc tgatcacact gttgcttatt
     5941 cacaatcagg catggaagtc tacagttggg gatggggtga ttttgggaga ttaggccatg
     6001 gtaactcaag cgacttgttt actccgctac caatcaaagc attgcacggt attcggatca
     6061 agcagattgc ttgtggggat agtcattgtt tggctgtcac tatggaagga gaggtccaga
     6121 gttggggccg caaccagaat ggtcaacttg gtctggggga caccgaagat tctctagtgc
     6181 ctcagaagat tcaagccttt gagggaatac gaatcaaaat ggttgctgct ggtgcagaac
     6241 acactgctgc agttacagaa gatggtgacc tctatggatg gggctgggga agatacggaa
     6301 atttgggatt aggtgaccgg actgaccgct tagttcctga aagagttacc tctactggtg
     6361 gtgagaaaat gtcaatggtt gcttgtggat ggcggcacac aatatcagtt tcctactctg
     6421 gagcattgta tacttatgga tggagcaaat atggacagct aggacatgga gacttggagg
     6481 atcaccttat tcctcacaaa ctggaagcac tgagcaacag ttttatctcc cagatttcgg
     6541 gaggttggag acatacaatg gcattgactt cagatggaaa actatatgga tggggttgga
     6601 ataagtttgg acaagtagga gtcggcaata atttagatca gtgttctcct gtgcaagtgc
     6661 gatttcccga tgatcagaaa gtagttcaag tctcatgtgg atggagacat accttggctg
     6721 tcactgaaag aaataacgtg tttgcttggg gtagaggtac aaatggacag ctcggcattg
     6781 gagagtcggt tgacaggaac tttcccaaga ttatagaggc actcagcgtc gatggagcaa
     6841 gtggacaaca tatagaatct tctaatatcg atccatcttc agggaaaagc tgggtgtcgc
     6901 ctgcagagag atatgcagtt gttcctgatg aaacgggcct aacggatggt tcaagcaaag
     6961 gtaatggagg tgatatcagt gttccacaaa ctgatgtcaa gcgtgtacga attccgcggg
     7021 cccgggatac tagttatccg tacgacgtac cagactacgc ataagaaaag tgaggatcct
     7081 gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc tattgtaaaa
     7141 ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg aagatgtccc
     7201 ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact ctgttgacaa
     7261 ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa ctttagcttg
     7321 catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa gtactttctc
     7381 taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc acagttttag
     7441 agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg
     7501 cgtggaaata ttcttattgg tagaaacaac tacatcctgg tcatcatcct gcctttctct
     7561 ttatggttac aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg
     7621 ggcccctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
     7681 ctggttgttg tgctgtctca tcattttggc aaaggattca ctcctcaggt gcaggctgcc
     7741 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     7801 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     7861 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     7921 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     7981 ttggcaacat atgccatatg ctggctgcca tgaacaaagg tggctataaa gaggtcatca
     8041 gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg
     8101 ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc
     8161 cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt
     8221 ccctcttctc ttatgaagat ccctcgagga gctttttgca aaagccctag gcctccaaaa
     8281 aagcctcttc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa
     8341 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     8401 gggatgggcg gagttagggg cgggactatg gttgctgact aattgctgca ttaatgaatc
     8461 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8521 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8581 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8641 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8701 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8761 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8821 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8881 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8941 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9001 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9061 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9121 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9181 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9301 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9361 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9421 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9481 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9541 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9601 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9661 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9721 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9781 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9841 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9901 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9961 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10021 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10081 agcagaactt taaaagtgct catcattgcc acctccacct gaaaacgttc ttcggggcga
    10141 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10201 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10261 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10321 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10381 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10441 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    10501 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
    10561 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
    10621 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
    10681 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
    10741 cgcatcagga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
    10801 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
    10861 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
    10921 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
    10981 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
    11041 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
    11101 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
    11161 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcgcgc cattcgccat
    11221 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    11281 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    11341 cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag g
//
