LOCUS       sequence-75111-e         921 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  mammalian expression of mouse Oct3/4 fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLPS-mOct4-gfp
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    216..249
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(344..363)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgcgaccggt agctgtggaa aaaaaaggga caggataagt atgatattgt caaggaaacc
       61 ctgaattcga agcttgagct cgagatccac gtcaggtggc acttttcggg gaaatgtgcg
      121 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      181 ataaccctga taaatgcttc aataatattg gatctataac ttcgtataat gtatgctata
      241 cgaagttatg gatctttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
      301 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
      361 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
      421 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
      481 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
      541 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
      601 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
      661 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
      721 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
      781 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
      841 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
      901 acaacagtac tgcgatgagt g
//
