LOCUS       sequence-75213-e         762 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Destination vector for Golden Gate assembly; 3' entry vector for 
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: p3E_GGWDest-
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     73..89
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        94..112
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    150..245
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(246..262)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     284..301
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    418..439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(454..459,460..477,478..484)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    492..508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     497..519
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     516..532
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        550..568
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     550..567
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      662..671
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      666..675
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(706..724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(731..748)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(731..747)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(740..762)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg
       61 ggccagagct gccaggaaac agctatgacc atgtaatacg actcactata ggggatatcg
      121 cggccgcgtt aacgctacca tggagctcca aataatgatt ttattttgac tgatagtgac
      181 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtataataa
      241 agttggatac cgtcgacctc gagccttaag agaccccgag cgcagcgagt cagtgagcga
      301 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      361 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      421 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      481 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
      541 cgccaagcta tttaggtgac actatagaat actcaagcta tgcatccaac gcgttgggag
      601 ctctcccata tggtcgacct gcaggcggcc gcgaattcac tagtgatatc gaattcccgc
      661 ggccgccatg gcggccggga gcatgcgacg tcgggcccaa ttcgccctat agtgagtcgt
      721 attacaattc actggccgtc gttttacaac gtcgtgactg gg
//
