LOCUS       sequence-75664-e         876 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral expression vector. Contains an altered Kozak sequence 
            and/or upstream open reading frames to modulate expression at the 
            level of translation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Ferreira JP, Overton KW, Wang CL
  TITLE     Tuning gene expression with synthetic upstream open reading frames.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 
            10.1073/pnas.1305590110. Epub 2013 Jun 24.
  PUBMED    23798422
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1305590110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-9- 9"; volume: "110"; issue: "28"; pages: "11284-9"
COMMENT     Sequence Label: pWPT-/UAA-mEGFP-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             144..854
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(183..204)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(444..463)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     791..812
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
       61 tttgccgcca gaacacaggt gtcgtgacgc gcatccaggc ctaagcttac gctcgagtaa
      121 ctaactaata aatgggcgaa ttcagcaagg gcgaggagct gttcaccggg gtggtgccca
      181 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      241 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      301 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
      361 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      421 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      481 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      541 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      601 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      661 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      721 tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga
      781 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      841 acgagctgta caagttcgaa taagcatgca tctagg
//
