LOCUS       sequence-75813-e         547 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Cumate-inducible expression of HA-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQH
FEATURES             Location/Qualifiers
     source          1..547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(72..137)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(189..215)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    240..267
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    310..337
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
ORIGIN
        1 gaattcaaaa aaaaggccgg gggagcgatc ccctggcctt tttttttgcg ggtcacttca
       61 ccggatcctc acttgtcgtc gtcatccttg tagtcgatgt cgtggtcctt atagtcgccg
      121 tcgtggtcct tgtagtccgg atcccaattg gagctcggta cctcgagatc ttctagagtc
      181 atattaatgg cgtagtccgg gacgtcgtac gggtacatgc ggaagcttcc tctactagtt
      241 acaaacagac cagattgtct gtttgttaac cggcgcagtt agcggcttcg cggcgatccg
      301 tcaaccatgt acaaacagac cagattgtct gtttgttccg gtacattgac atatgtatcc
      361 gctcatgata caataaccct gatgctagca gggagagacc ccgaatggtc atcatgtcgc
      421 cgaagcgccg cacccaggcc gagcgcgcga tggaaaccca gggcaagctg atcgccgccg
      481 ccctgggcgt cctgcgcgag aagggctatg ccggcttccg catcgccgac gtcccgggcg
      541 ccgccgg
//
