LOCUS       sequence-75930-e        1063 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  expresses GAL4BD-Rab4BQ67LdeltaCGCin yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Cortes C, Rzomp KA, Tvinnereim A, Scidmore MA, Wizel B
  TITLE     Chlamydia pneumoniae inclusion membrane protein Cpn0585 interacts 
            with multiple Rab GTPases.
  JOURNAL   Infect Immun. 2007 Dec;75(12):5586-96. Epub 2007 Oct 1.
  PUBMED    17908815
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2007-12"; volume: "75(12)"; pages: "5586-96. Epub 
            2007 Oct 1"
COMMENT     Sequence Label: GBK-Rab4BQ67LdeltaGCG
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             52..81
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      762..809
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      836..1023
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(891..915)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tcgccggaat ttgtaatacg actcactata gggcgagccg ccatcatgga ggagcagaag
       61 ctgatctcag aggaggacct gcatatggcc atggaggccg aattcatggc tgagacctag
      121 gacttcctct tcaaattcct ggtgattggc agtgcaggaa ctggcaaatc atgtctcctt
      181 catcagttca ttgagaataa gttcaaacag gactccaacc acacaatcgg cgtggagttt
      241 ggatcccggg tggtcaacgt gggtgggaag actgtgaagc tacagatttg ggacacggct
      301 ggcctggagc ggtttcggtc agtgacgcgg agttattacc gaggggcggc tggagccctg
      361 ctggtgtacg acatcaccag ccgggagaca tacaactcac tggctgcctg gctgacggat
      421 gcccgcaccc tggccagccc caacatcgtg gtcatcctct gtggcaacaa gaaggacctg
      481 gaccctgagc gggaggtcac tttcctggag gcctcccgct ttgcccagga gaatgagctg
      541 atgttcctgg agaccagcgc tctcacaggc gagaacgtgg aggaggcgtt cctcaagtgt
      601 gcccgcacta tcctcaacaa gattgactca ggcgagctag acccggagag gatgggctct
      661 ggcattcagt acggggatgc gtccctccgc cagcttcggc agcctcggag tgcccaggcc
      721 gtggcccctc agggctgact cgacctgcag cggccgcata actagcataa ccccttgggg
      781 cctctaaacg ggtcttgagg ggttttttgc gcgcttgcag ccaagctaat tccgggcgaa
      841 tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa
      901 attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct
      961 gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac
     1021 cggcatgcaa gcttggcgta atcatggtga tagctgtttc ctg
//
