LOCUS       sequence-75956-e         838 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses thiol redox-sensitive ratiometric sensor roGFP in the 
            mitochondrial intermembrane space of mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Waypa GB, Marks JD, Guzy R, Mungai PT, Schriewer J, Dokic D, 
            Schumacker PT
  TITLE     Hypoxia triggers subcellular compartmental redox signaling in 
            vascular smooth muscle cells.
  JOURNAL   Circ Res. 2010 Feb 19;106(3):526-35. doi: 
            10.1161/CIRCRESAHA.109.206334. Epub 2009 Dec 17.
  PUBMED    20019331
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1161/CIRCRESAHA.109.206334"; journalName: "Circ Res"; date: 
            "2010-02-19- 19"; volume: "106"; issue: "3"; pages: "526-35"
COMMENT     Sequence Label: GPD-roGFP
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..31
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15..31
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(36..749,750..752,753..755))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     430..449
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     689..710
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(756..782)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gctgtggcgg ccgctctaga actagtggat ccttcttact tgtacagctc gtccatgccg
       61 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
      121 gggtctttgc tcagggcgga gcaggtgctc aggtagtggt tgtcgggcag cagcacgggg
      181 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg
      241 atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata
      301 tagacgttgt ggcagttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
      361 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
      421 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
      481 ccttcgggca tggcggactt gaagaagtcg tgccgcttca tgtggtcggg gtagcggctg
      541 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
      601 ccggtggtgc tgatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      661 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      721 accccggtga acagctcctc gcccttgctc accattaagc ttgcataatc aggaacatca
      781 taatcgatgt ctggaggcag caggctgatt tcagtactat ctgtgagttg ttctgttc
//
