LOCUS       sequence-76090-e         951 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat1
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    76..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             115..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             148..192
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             208..225
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat
       61 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      121 taactttaag aaggagatat acatatgaaa gaaaccgctg ctgctaaatt cgaacgccag
      181 cacatggaca gcccagatct gggtaccctg gtgccacgcg gttccatggc gatatcggat
      241 cgggaaggtg tcaatgataa cgaagaaggc tttttcagcg ctcgcaacta tctgggcatc
      301 gacggcatcc cggaatttgg ccgcgtggcg gctgattttc tggcgaaaag cgttttcgac
      361 accctggcaa cggcagcaaa ggtcctgctg cagctggcac tgacccaaac gaaactgggc
      421 ctgattgaag tgcaggcgcc gatcctgagc cgttctaccg ttgaagccat ttgggcaggt
      481 atcaagtcct atggcgtggg taacgccccg cagaataaag catttctgca ggaactgcaa
      541 gaagcaagcg atcgtatttc tgacatcccg gaattcgttc gcattgcggt ctacagctct
      601 ctgattaaac tgtctatcgt tgatgtcaac gccggcgcac agccgctgta taatcagcaa
      661 aaacaagccc tggaagacag tgtgaagtac attgaagctc tgtccgcgat cgtcgaaacc
      721 ggccagaaag tgagcccgga aatctttagt acgtccctgc gtaccgttac ggcggatatt
      781 ccgatcgtcg gtgacgctcg cctgtctgaa gcgctggaac aagtgcgtct ggtggatgtt
      841 ctgcgcctgg acgccttcct ggcaagtatt cgtgatgaac attcctggcg catcgtgctg
      901 ctggataacc agcgttcagt tgacctgctg ttttcgcaac tgaacaaaca c
//
