LOCUS       sequence-76302-e        9936 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses enzymatically monobiotinylated full length SERA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9936)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 9936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: SERA2-bio-His
FEATURES             Location/Qualifiers
     source          1..9936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5100..5117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5169..5224
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5223..5242)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7460..7478)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7546..7650
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7651..7719,7720..8511)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7869..7888)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8682..9270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9171..9190
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9424..9441
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9558..9579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9594..9599,9600..9617,9618..9624)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9632..9648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9637..9659
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9656..9672
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9656..9672
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcag
     1201 agacaaccac agatgagtct gccaccggca gcctgagcag cgacggcagc agagtgacaa
     1261 cccaggccag aatcgagaag cccaagcagc agcccaccct gcctacactg gcccaggaaa
     1321 cacagcctca acaacagcag cagcagaaag aagtgggcag cggcatcgga gccgagcaga
     1381 aggtggaatc tgccagacct ggcgccgagg tgtcccagag cgacgtggaa agagccggca
     1441 gatcttctgg cacaggcggc agcgtgggca ccaagatctc tccaggatct cagggccagg
     1501 gcaaggtggc aggacctcag ctgcctagac tgccacagct gccccagagc ttcgagcagt
     1561 ccagaaacca gcagagcagc cccgtgaccc ccaagagaaa cggcatcagc cccaccaacg
     1621 ccaagagccc tgagagcgtg ctgcctcctg cccagtcctt caccaacctg aacaaggcca
     1681 ccatccccca caccatccct atcaagagca gcttcctgaa gtactacaag ggcgtgaaga
     1741 tcacaggcag ctgcggcgtg cagttccagc tggtcattgt gccccacctg ttcatctacg
     1801 tcgagacaaa agagaacaac atccagctcg agccccggtt catgaagctg aacgagcgga
     1861 tcgacttcga gaaggacaag agcaacctga agaacaagtg cgacgtgaac aagaagcaga
     1921 gcttcaagtt catcctgtac ctgcagcacg acctgatcac catcaagtgg aaggtgtacg
     1981 aggaaaagcc cgacaccacc accagaatcg ctcccggaca ggtggacctg aacgtggacg
     2041 tgaagcggta caagctgccc aagctggacc agcccgtgat cagcatccag atccacagcc
     2101 tggctcagga cggcgagaca tacctgatgg aaagcaagga ctacaacctc gaggaccaga
     2161 tccccgagaa gtgcgaggct atcgccagcg actgcttcct gagcggcaac gtggacatcg
     2221 agaagtgtct gcagtgcacc ctgctggtca tcaaggccga caagaacgac gagtgcctga
     2281 aatacgtgtc caagaacgtg aaggaccgct tcgaggaaat cctgaccaag ggcgaggacg
     2341 acgccgacag cgacgagtac gactttatcg cccctgccaa ctacatcctc aagaacatct
     2401 acaagaagaa tgacgccaac ggcaagaaag agctgctgca cttcaaggac atcaacaaca
     2461 atctgaagct cgaactgatc aactactgca acctgctgaa ggacaacgac gtgtccggca
     2521 tcctgaccta cgagaagctg ggcaacgtgc aggacatctt caacaacctg gccaagctgc
     2581 tcgaggaaca caaagaagaa aacaactacg tcctgtacca caagatgaag aacgaggtgc
     2641 tgtgcctgaa gaatgccaac gactggatga agaacaaagc cggcctggtg ctgccccagc
     2701 tgaagtacag cctgaacaaa ttcaacaaaa acaaagagaa ttacatcaaa gaaaacatct
     2761 ttgaagagga cgagaacggc atcgtggacc tcaccaagtt ccccgtggac accagctaca
     2821 gcagctacaa ctacgccgac tccctgtact gcaacaggga atactgcaat cgcctcaagg
     2881 accacaacaa ttgcatcagc aagatcaacg tcgaggatca gaagaactgc gccctgagct
     2941 gggccttcgc cagcatctac cacctggaaa caatcaagtg catgaagggc tacgagcccc
     3001 tgaacgccgc cgtgctgtac gtgaccaact gcctgaaaaa caagaacaac gatgtgtgca
     3061 ccgagggcag caaccccctg gtgttcctgg aaactatcga agagaagggc ttcctgccca
     3121 ccgagagcaa ctacccctac gaccagagca aagtgggcga cgtgtgcccc caggtgcaga
     3181 acgactggga caacgtgttc gagaacacca aagtgctcga gtacaacaac gcccccttca
     3241 gcgtgggaac aaagggctat atcgcctacg agagcgaagt gttccagaag gacatcgaca
     3301 gcttcgtgaa gctgatcaag gacgagatca tgaacaaggg cagcgtgatc gcctatgtga
     3361 aggccaagaa tgtgctgggc tatgagctga acggcaaaaa ggtccagaac ctgtgcggcg
     3421 acaagaaacc cgaccacgcc gtgaacatcg tgggctacgg caactacatt aacaacaagg
     3481 gggagaagaa atcctactgg atcgtgcgga actcctgggg caagtactgg ggcgacgacg
     3541 gctacttcaa agtggatatg tacggccctc ctacctgcga ggacaacttc atccacagcg
     3601 tggtcgtgtt caacgtggaa gtgcccgtga acgagaactt cgacaaaaaa gagcacgata
     3661 tctacaagtc ctatctgaag aactcccccg acttctacca caacatctac tacaagaact
     3721 acaatttcga gaactacgtg tcccccatca gcacttggag cgaggtgctc cacaacaccc
     3781 tgtataacaa caacctcatc tggggccagg aaaccaccga ccctatcggc gagggaaagc
     3841 tgcctgcctc tgaggccgga cacctgagag gcgagggcaa gggcaacagc gaggaaaacc
     3901 agggccagcg gagcaacgct aacggcgcca tcagcagcaa tcaggccacc ggcggcaaga
     3961 tcaccaagca gggcgagagc aaggacgacg gcgtggcact gcctgtgtcc accgatggca
     4021 agcccaatac cgccagcagc gtggtggata ccaacgacca gcggtccctg cccaacccta
     4081 gagccacatc tctgcagccc cctagcgtgc agatccccaa tcacgagggc acaagcgccc
     4141 caggcaacag cagaacccct agcatcgtgt ccccaacagc cgccgagaag tctagaaagg
     4201 cccagatctt ccacgtgctg aagcacatca agaacagccg gatcaagatg ggcctcgtga
     4261 agtacgacaa cagcgacaac atcggcggcg accacgtgtg cagcagaaca tatgccgtga
     4321 accctgagaa gcaggaagag tgcgtcaagt tctgcgaaga gaactgggag aactgcaaga
     4381 acaagcccag ccccggctac tgcctggcca aactcaagaa taccaatgag tgcttcttct
     4441 gctacgtggg cgcgccgtcg acctccatca cggcctataa gagtgagggg gagtcagcgg
     4501 agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg agatggaagg
     4561 cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac caaaaggtgt
     4621 ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc ccactcaccc
     4681 ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc ctgactctgg
     4741 acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact cagcccgaca
     4801 gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga ctgatcttga
     4861 agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa gtgcaggccc
     4921 ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag atggactcca
     4981 agatccaggt tttatccaaa gggttgaatt ccggcagcct gcaccacatc ctggacgccc
     5041 agaagatgct gtggaaccac agggacagga acctgcctcc tctggcccct ctgggccccc
     5101 accaccatca ccaccattga gtctagacac gtgattaatt aaggatcccc cgacctcgac
     5161 ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     5221 ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc ggagatctct
     5281 agctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     5341 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     5401 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     5461 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     5521 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     5581 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     5641 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     5701 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     5761 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     5821 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     5881 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     5941 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     6001 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     6061 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     6121 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     6181 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     6241 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     6301 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     6361 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     6421 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     6481 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     6541 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     6601 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     6661 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     6721 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     6781 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     6841 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     6901 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     6961 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     7021 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     7081 tgctatccta atagagatta gggtagtata tgctatccta atttatatct gggtagcata
     7141 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcatatgct
     7201 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     7261 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     7321 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     7381 atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca aacatgagaa
     7441 ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     7501 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7561 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7621 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     7681 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     7741 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7801 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     7861 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     7921 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7981 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8041 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8101 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8161 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     8221 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8281 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     8341 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     8401 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     8461 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     8521 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     8581 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     8641 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     8701 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8761 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8821 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8881 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8941 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9001 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9061 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9121 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9181 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9241 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9301 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     9361 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     9421 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     9481 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     9541 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     9601 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     9661 aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt ctcatgtttg
     9721 acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt
     9781 aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
     9841 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
     9901 tgtacattta tattggctca tgtccaatat gaccgc
//
