LOCUS       sequence-76312-e        9888 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses enzymatically monobiotinylated full length RIPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 9888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: RIPR-bio-His
FEATURES             Location/Qualifiers
     source          1..9888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5052..5069
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5121..5176
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5175..5194)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7412..7430)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7498..7602
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7603..7671,7672..8463)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7821..7840)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8634..9222
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9123..9142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9376..9393
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9510..9531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9546..9551,9552..9569,9570..9576)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9584..9600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9589..9611
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9608..9624
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9608..9624
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 tcgatctgat cgagggcatc ttctacgaga agaacgagat cgacaagctg accttcagcc
     1261 tggaccacag agtgcgggac aacctgaaaa ccgacctgat cctgaacaac aacggcgaga
     1321 acgactacgc ctacctgaac aaatacgtgt acaccatcct gaaccgggac agcaccgaga
     1381 agatcaagac cttcttcagc cacaacaagg acatgaagtc ctgcgactac ttcatcagca
     1441 aagagtacaa cagcgccgac aagaccaacc agatctgcta caagaaaacc ttctgcggcg
     1501 tcgtgatccc caacagcgag gaaatcaaga caaacaagat caccaacgat aagctgtact
     1561 gcgcccactt caactccgcc cacatcatca tctactacat cagccagccc ctgctgctgg
     1621 aaccccacgt ggtgtacgag gaaaccttct tcgagaaggg caagaacgac cagatcaact
     1681 gccagggcat gtacatctcc ctgcggagtg tgcatgtgca cacccacaac gccatcctgc
     1741 agcaggaaac cctgacctac atcaagaacc tgtgcgacgg caagaacaac tgcaagttcg
     1801 acttcgacag cattaagtac gagaacaagg ccctgaccca ctacctgttc ttcatcaaca
     1861 tccagtacca gtgcatcagc cccctgaacc tgcaggaaaa cgagatgtgc gacgtgtaca
     1921 acgacgatac ccacaaggcc acatgcaaat acggcttcaa caagatcgag ctgctgaaga
     1981 atgtgtgcga agagaactac cggtgcaccc aggacatctg cagcgtgaac cagttctgcg
     2041 acggggagaa cgaggcctgt acctgcaaga ccagcctgct gcccagcgcc aagaacaatt
     2101 gcgagtacaa cgatctgtgc accgtgctga actgccccga gaactctgcc tgcgagcaga
     2161 tcggcaacgg caaaaaggcc gagtgcaagt gcgagaacgg caagtactac cacaacaaca
     2221 agtgctacac caagaacgat ctggaactgg ccatcaagat tgagccccac aagaaagaga
     2281 agttctataa gaacaacctg taccagggca aagccctgaa gcccgagtac atcttcatgc
     2341 agtgcgaaaa cgggttcagc atcgaagtga tcaacgccta cgtgtcctgc taccgggtgt
     2401 ccttcaatct gaacaagctg aaatacgtga ccgagagcct gaagaaaatg tgtgatggca
     2461 agaccaagtg cgcctacggc aacaccatcg accccatcga cgacctgaac caccacaaca
     2521 tctgcaacaa cttcaatacc atcttcaaat acgactacct gtgcgtgttc aacaaccaga
     2581 atatcgcctc cgacaagaac agccatctgc acagcaacat ccccagcctg tacaactccg
     2641 ctatcctgcc cgacatcaac aaggccaagt tccacctgat cagccggaac agccgcacca
     2701 accagtaccc ccacaacaat atcgccatgc tggaaatcca gaatgagatc agctcccaca
     2761 acagcaacca gtttagcacc gacccccaca ccaactctaa caatatcaac aacatgaaca
     2821 ttaagaaggt ggaaatcttc cgcagccggt tcagcagcaa gctgcagtgt cagggcggca
     2881 agatcaatat cgataaggcc attctgaagg gcggcgaggg ctgcaatgat ctgctgctga
     2941 ccaacagcct gaagtcctac tgcaacgacc tgagcgagtg cgacatcggc ctgatctacc
     3001 acttcgacac ctactgcatc aatgaccagt acctgttcgt gtcctacagc tgcagcaacc
     3061 tgtgcaacaa gtgtcacaac aacgccgcct gctacggcaa tcggttcaac tacgactgct
     3121 tctgcgacaa cccctacatc tctaagtatg gcaacaagct gtgcgagcgg cccaacgact
     3181 gcgagagcgt gctgtgtagc cagaaccaag tgtgccagat cctgcccaat gacaaactga
     3241 tctgccagtg tgaagagggg tacaagaacg tgaagggcaa atgcgtgccc gataacaagt
     3301 gcgacctgtc ctgccccagc aacaaagtgt gcgtgatcga gaacggaaag cagacctgca
     3361 agtgcagcga gagattcgtg ctggaaaacg gcgtgtgcat ctgcgccaac gactacaaga
     3421 tggaagatgg catcaactgc attgccaaaa acaagtgcaa gcggaaagag tatgagaata
     3481 tctgcaccaa ccccaacgaa atgtgtgcct acaacgaaga gacagacatc gtgaagtgcg
     3541 agtgcaaaga gcactactac cggtccagca gaggcgagtg catcctgaat gactactgca
     3601 aggacatcaa ttgcaaagaa aacgaggaat gcagcatcgt gaacttcaag cctgagtgcg
     3661 tgtgtaaaga gaatctgaag aagaacaaca agggggagtg catctatgag aacagctgcc
     3721 tgatcaacga gggcaactgc cccaaggaca gcaagtgtat ctacagagag tacaagcccc
     3781 acgagtgcgt gtgcaacaag cagggacacg tggccgtgaa cgggaagtgc gtgctggaag
     3841 ataagtgcgt gcacaacaag aagtgtagcg agaactccat ctgcgtgaac gtgatgaaca
     3901 aagaacctat ctgtgtgtgc acctacaact actacaaaaa agatggcgtg tgcctgatcc
     3961 agaatccctg cctgaaggac aacggcggct gctccagaaa cagcgagtgc accttcaagt
     4021 acagcaagat taactgcgcc tgcaaagaga attacaagaa caaggacgac agctgcgtgc
     4081 ccaacaccaa cgagtacgac gagagcttca ccttccagta taacgacgac gccagcatca
     4141 tcctgggcgc ctgcggcatg atcgagttca gctacatcta caatcagatc atctggaaga
     4201 ttaacaacgc caaagaaagc tacgtgttct actacgatta ccccaccgcc ggcaacatcg
     4261 aggtgcagat caagaacgaa atctttcaca caatcatcta cctgaaaaag aaaatcggca
     4321 acagcgtgat ctacgacgac ttccaggtgg accaccagac ctgtatctac gagaacgtgt
     4381 tctattactc caaccagaat ggcgcgccgt cgacctccat cacggcctat aagagtgagg
     4441 gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg cagggagagt
     4501 tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc tccctaaaga
     4561 accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg tccgaaacgc
     4621 tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct ggcaacctga
     4681 ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg atgaaagtga
     4741 ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca cccaagatga
     4801 gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag aaagtgattc
     4861 aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt gaagaggtca
     4921 agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc ctgcaccaca
     4981 tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct cctctggccc
     5041 ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa ttaaggatcc
     5101 cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     5161 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc
     5221 tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac
     5281 atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt
     5341 gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct
     5401 gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca
     5461 tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc
     5521 ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac
     5581 tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc
     5641 tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca
     5701 tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt
     5761 acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg
     5821 atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg
     5881 gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact
     5941 ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga
     6001 gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca
     6061 gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct
     6121 cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac
     6181 aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt
     6241 tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt
     6301 cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa
     6361 agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta
     6421 aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga
     6481 aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa
     6541 aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt
     6601 gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa
     6661 gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc
     6721 aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt
     6781 tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     6841 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     6901 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     6961 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     7021 ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat
     7081 ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     7141 gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
     7201 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg
     7261 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     7321 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt
     7381 caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7441 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     7501 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7561 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7621 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7681 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7741 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7801 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     7861 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7921 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7981 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8041 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8101 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     8161 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8221 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8281 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8341 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8401 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8461 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8521 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8581 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8641 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8701 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8761 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8821 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8881 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8941 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9001 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9061 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9121 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9181 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9241 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9301 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9361 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9421 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     9481 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     9541 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     9601 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgaccaa
     9661 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
     9721 cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat
     9781 tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
     9841 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgc
//
