LOCUS       sequence-76441-e         761 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression of a GFP-dependent transcription factor component in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Tang JC, Szikra T, Kozorovitskiy Y, Teixiera M, Sabatini BL, Roska 
            B, Cepko CL
  TITLE     A nanobody-based system using fluorescent proteins as scaffolds for 
            cell-specific gene manipulation.
  JOURNAL   Cell. 2013 Aug 15;154(4):928-39. doi: 10.1016/j.cell.2013.07.021.
  PUBMED    23953120
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.07"; journalName: "Cell"; date: "2013-08-15- 
            15"; volume: "154"; issue: "4"; pages: "928-39"
COMMENT     Sequence Label: pCAG-GBP1-10gly-Gal4DBD
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..23
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(112..552)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 aatttttggc agagggaaaa agatctcagt ggtatttgtg agccagggca ttggccacac
       61 cagccaccac cttctgatag gcagcctgca cctgaggagt gcggccgctt acgatacagt
      121 caactgtctt tgacctttgt tactactctc ttccgatgat gatgtcgcac ttattctatg
      181 ctgtctcaat gttagaggca tatcagtctc cactgaagcc aatctatctg tgacggcatc
      241 tttattcaca ttatcttgta caaataatcc tgttaacaat gcttttatat cctgtaaaga
      301 atccattttc aaaatcatgt caaggtcttc tcgaggaaaa atcagtagaa atagctgttc
      361 cagtctttct agccttgatt ccacttctgt cagatgtgcc ctagtcagcg gagacctttt
      421 ggttttggga gagtagcgac actcccagtt gttcttcaga cacttggcgc acttcggttt
      481 ttctttggag cacttgagct ttttaagtcg gcaaatatcg catgcttgtt cgatagaaga
      541 cagtagcttc atactagtac ctccacctcc acctccacca cctcctccgc tagcagaact
      601 aacagtcact tgtgtgccct gtccccagta ttcaaagccc acattgacgt tggagtagta
      661 cacagcagtg tcctcaggtt tcagggagtt catctggagg tacacggtgt ttctagcatc
      721 gtccctacta atggtgaacc gtcctttcac gctatcctcg t
//
