LOCUS       sequence-76483-e         641 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  3rd generation Lentiviral vector with bi-cistronic expression of 
            dtomato and luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Fujiwara T, Yakoub MA, Chandler A, Christ AB, Yang G, Ouerfelli O, 
            Rajasekhar VK, Yoshida A, Kondo H, Hata T, Tazawa H, Dogan Y, Moore 
            MAS, Fujiwara T, Ozaki T, Purdue E, Healey JH
  TITLE     CSF-1/CSF-1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms 
            Tumor-Associated Macrophages and Stimulates T-Cell Infiltration in 
            the Sarcoma Microenvironment.
  JOURNAL   Mol Cancer Ther. 2021 Jun 4. pii: 1535-7163.MCT-20-0591. doi: 
            10.1158/1535-7163.MCT-20-0591.
  PUBMED    34088832
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Ther. 2021 Jun 4. pii: 1535-7163.MCT-20-0591. doi: 
            10.1158/1535-7163.MCT-20-0591."
COMMENT     Sequence Label: pUltra-Chili-Luc
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..101)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(194..217)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(392..411)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatac tagaaggccc gggattctcc
       61 tccacgtcac ctgcttgttt gagtagtgag aagtttgttg ctccagatcc cttgtacagc
      121 tcgtccatgc cgtacaggaa caggtggtgg cggccctcgg agcgctcgta ctgttccacg
      181 atggtgtagt cctcgttgtg ggaggtgatg tccagcttgg tgtccacgta gtagtagccg
      241 ggcagttgca cgggcttctt ggccatgtag atggtcttga actccaccag gtagtggccg
      301 ccgtccttca gcttcagggc ctggtggatc tcgcccttca gcacgccgtc gcgggggtac
      361 aggcgctcgg tggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcgggg
      421 gggaagttgg tgccgcgcat cttcaccttg tagatcagcg tgccgtcctg cagggaggag
      481 tcctgggtca cggtcaccag accgccgtcc tcgaagttca tcacgcgctc ccacttgaag
      541 ccctcgggga aggacagctt cttgtaatcg gggatgtcgg cggggtgctt cacgtacgcc
      601 ttggagccgt acatgaactg gggggacagg atgtcccagg c
//
