LOCUS       sequence-76543-e         735 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression of a GFP-dependent transcription factor component in 
            mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Tang JC, Szikra T, Kozorovitskiy Y, Teixiera M, Sabatini BL, Roska 
            B, Cepko CL
  TITLE     A nanobody-based system using fluorescent proteins as scaffolds for 
            cell-specific gene manipulation.
  JOURNAL   Cell. 2013 Aug 15;154(4):928-39. doi: 10.1016/j.cell.2013.07.021.
  PUBMED    23953120
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.07"; journalName: "Cell"; date: "2013-08-15- 
            15"; volume: "154"; issue: "4"; pages: "928-39"
COMMENT     Sequence Label: pCAG-p65AD-GBP6
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      112..121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             148..168
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             358..729
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
ORIGIN
        1 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
       61 ttggcaaaga attctgcagt cgacggtacc gcgggcccgg gatccaccgg tgccaccatg
      121 gataaagcgg aattaattcc cgagcctcca aaaaagaaga gaaaggtcga attgggtacc
      181 ccttctgggc agatcagcca ggcctcggcc ttggccccgg cccctcccca agtcctgccc
      241 caggctccag cccctgcccc tgctccagcc atggtatcag ctctggccca ggccccagcc
      301 cctgtcccag tcctagcccc aggccctcct caggctgtgg ccccacctgc ccccaagccc
      361 acccaggctg gggaaggaac gctgtcagag gccctgctgc agctgcagtt tgatgatgaa
      421 gacctggggg ccttgcttgg caacagcaca gacccagctg tgttcacaga cctggcatcc
      481 gtcgacaact ccgagtttca gcagctgctg aaccagggca tacctgtggc cccccacaca
      541 actgagccca tgctgatgga gtaccctgag gctataactc gcctagtgac aggggcccag
      601 aggccccccg acccagctcc tgctccactg ggggccccgg ggctccccaa tggcctcctt
      661 tcaggagatg aagacttctc ctccattgcg gacatggact tctcagccct gctgagtcag
      721 atcagctccg ctagc
//
