LOCUS       sequence-76552-e         992 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Cumate-inducible expression of lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQF-lacZ
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..93
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     primer_bind     120..136
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     120..136
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             132..305
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(152..169)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(152..168)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(161..183)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acagacaatc tggtctgttt gtacatggtt gacggatcgc cgcgaagccg ctaactgcgc
       61 cggttaacaa acagacaatc tggtctgttt gtaactagag gtccggatcc cggtacccac
      121 aggaaacagc tatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact
      181 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      241 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      301 gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt
      361 gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg
      421 atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg tttgttccca
      481 cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg
      541 aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg tggtgcaacg
      601 ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac ctgagcgcat
      661 ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt gacggcagtt
      721 atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc
      781 ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat gatgatttca
      841 gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac tacctacggg
      901 taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg
      961 gtgaaattat cgatgagcgt ggtggttatg cc
//
